维生素C对海马神经细胞生长影响的研究  

THE EFFECT OF VITAMIN C ON THE GROWTH OF HIPPOCAMPAL NEURONS IN VITRO

作　　者：洪燕[1] 肖锦腾[1] 李树田[1] 王福庄[1] 丁爱石[1] 要航[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,军事医学科学院基础医学研究所

出　　处：《营养学报》1995年第1期7-11,共5页Acta Nutrimenta Sinica

基　　金：军队八五规划课题

摘　　要：本研究用新生大鼠脑海马区细胞进行体外培养，观察不同浓度（１０－３～１０－６ｍｏｌ／Ｌ）维生素Ｃ（ＶＣ）对海马细胞生长的影响。结果表明，培养１４天的细胞存活数在１０－６ｍｏｌ／Ｌ和１０－５ｍｏｌ／ＬＶＣ组与对照组（培养液中不加ＶＣ）之间无明显差别，１０－４ｍｏｌ／ＬＶＣ组明显高于对照组，而１０－３ｍｏｌ／ＬＶＣ组存活细胞很少。进一步观察可见１０－４ｍｏｌ／ＬＶＣ组细胞突起数目、长度、胞体面积和周长均高于对照组（Ｐ＜０．０５），细胞总蛋白相对含量明显增加。结果提示，１０－４ｍｏｌ／ＬＶＣ对体外培养海马神经细胞的生长有一定促进作用。he effect of vitamin C(VC)on the growth of hippocampal neurons from newborn rats was investigated by using dissociated cell culture。 Con-centrations of VC in culture medium was 10-6 mol/L, 10-5 mol/L, 10-4mol/L and 10-3 mol/L respectively,On the 14th day, the numbers of survival neu- rons in 10-6mol/L and 10-5 mol/L VC groups were almost the same as contro(no VC in medium).The numbers of survival neurons in 10-4 mol/L VC gro- up were significantly higher than those of control group(P<0.05),and sur- vival neurons in 10-3mol/L VC group were very scanty,The number and length of neuronal processcs as well as the area and perimeter of neurosoma in 10-4mol/L VC group were all increased markedly as compared with tho- se of control group.The total protein content in 10-4 mol/L VC group was also enhanced significantly as compared with that in control. These results indicated that the suitable amount of VC supplementation could improve neuronal growth in vitro。

关 键 词：海马 神经细胞 体外培养 维生素C 

分 类 号：R977.23[医药卫生—药品]