粉叶小檗愈伤组织的培养及小檗碱的含量  

CALLUS CULTURE OF BERBERIS PRUINOSA AND PERBERING CONTENT IN CALLUS

作　　者：李启任[1] 杨峻芸 曹安飞 赵梅 

机构地区：[1]云南大学生物系

出　　处：《云南植物研究》1995年第3期325-330,共6页Acta Botanica Yunnanica

基　　金：云南省教委基金

摘　　要：由粉叶小檗（Ｂｅｒｂｅｒｉｓｐｒｕｉｎｏｓａ）的茎、腋芽、叶、实生苗的子叶及胚轴均可诱导出愈伤组织。Ｂ＿５＋２，４－Ｄ０．５ｍｇ／Ｌ＋ＫＴ０，２ｍｇ／Ｌ培养基对诱导较好，而Ｂ＿５＋２，４－Ｄ０．５ｍＲ／Ｌ＋ＫＴ０．５ｍｇ／Ｌ对愈伤组织生长较适宜。接种量在０．４－０．８ｇ（２０ｍＬ培养基）之间较为适宜。经薄层层析一分光光度法、薄层扫描法鉴定，证明愈伤组织具有合成小檗碱的能力，含量高达１．８１４８％，接近原植物体含量（茎，１．５８％）。Form the excised stem, axillary bud, leaf cotyledon, plumulax axis of BerberisWuinosa Franch the callus could been induced on Gamborg(B_5) medium or Murashige-Skoog(MS) me-dium,but B_5 was better than MS, The combination of 2,4-D0.5 mg/L and KT 0.2 mg/L was found most effective for induction,while the combination of 2,4-D0.5 mg/L and KT 0.5 mg/ L was better to growth and alkaloids synthesis. The opitimum inoculum quantities for callus growth was about 0.4-0.8g/20 mL media。Identification by thin-layer chromatography,ultraviolet and visible absorption spectrum of alcohol extracts of callus and stem,leaves of B. pruinosa Franch. proved that the callus had the capability to synthesize berberine。 The contents of berberine in the callus was 1.8148 %, it is slightly higher than the contents of berberine in the stems of infact plants(it was 1.58%)。The growth curves of the callus are sigmoid. When the callus had cultured for about 27 days, the growth rate and contents of berberine were highe than other times。

关 键 词：粉叶小檗 小檗碱 愈伤组织 组织培养 

分 类 号：Q949.746.8[生物学—植物学]