六棱大麦“Frances”游离小孢子的分离、培养与植株再生  

In-vitro culture of isolated microspores and plant regeneration in six-row barley (Hordeum vulgare L.) cv.Frances

作　　者：项友斌[1] 刘静[1] 

机构地区：[1]浙江省农业科学院生物技术中心,杭州310021

出　　处：《浙江农业学报》1995年第3期182-186,共5页Acta Agriculturae Zhejiangensis

摘　　要：大麦小孢子单核中期的穗，经4℃预处理4星期后，直接剪成小段，捣碎、过滤、离心和洗涤后收集小孢子。用MSm＋Maltose60.0g／L＋1．0mg／LBAP对小孢子慢速振荡培养，7天后可观察到小孢子分裂的小细胞团，经3星期左右培养，小细胞团总数达最高，其频率为总小孢子的2．36％。这些小细胞团80％发育为类似体细胞胚结构，20％为典型愈伤组织，类似体细胞结构胚置于1／2MS＋1.0mg／LBAP＋0．2mg／LNAA＋0．2mg／LZT分化培养基上，总分化频率为67％，其中绿苗率为39％，愈伤组织经胚性改造后，在1／2MS＋4．0mg／LBAP＋0．5mg／LNAA＋0.5mg／LIAA培养基上分化，总分化频率为62％，绿苗率为18％。Microspores were isolated from the spike of the six-row barley (Hordeum vulgare L,) cultivarFrances at the mid-unicellular pollen stage, pretreated at 4℃ for 4 weeks, by directly cutting the spike intosmall segments and homogenizing. The isolated microspores were incubated in MSm medium supplementedwith 60 g/ L maltose, 1.0mg/ L BAP with slowly shaking at 25℃ in darkness. Cell aggregatesfrom microspores were observed after 7 days from in initial culturing and the number of them reached tothe highest point at a frequency of 2.36% after about 3 weeks of culture. The cell aggregates developedembryo-like structure and call us at the rate of 80% and 20%, respectively. The embryo-like structureson 1/2 MS containing 1.0mg/ L BAP, 0.2mg/ L NAA and 0.2mg/ L ZT regenerated green plantlets(39%) and albino plantlets (28%). The calli by being improved on L3 medium with 2.0 mg / L 2, 4-D,0.1mg / L ABA and 0.2mg / L ZT, regenerated green plantlets (18%) and albino plantlets (44%), on 1 / 2MS medium by adding 4.0 mg / L BAP, 0.5mg / L N AA and 0.5mg/ L 1AA.

关 键 词：六棱大麦 游离小孢子 细胞团 植株再生 大麦 

分 类 号：S512.303.5[农业科学—作物学]