金顶侧耳多糖PC-4的结构确定与抗肿瘤活性的研究  

STRUCTURAL DETERMINATION AND STUDY ONANTITUMOR ACTIVITY OF POLYSACCHARIDEPC-4 FROM PLEUROTUSCITRIN OPILEATUS

作　　者：张丽萍[1] 苗春艳[1] 许丽艳[2] 张翼伸[1] 

机构地区：[1]东北师范大学生物系,长春130024 [2]白求恩医科大学应用基础研究所

出　　处：《真菌学报》1995年第1期69-74,共6页

基　　金：国家自然科学基金

摘　　要：从3％三氯乙酸浸提过的金顶侧耳(Pleurotus citrinopileatus)子实体中分离纯化另一水溶性多糖PC-4。该多糖分子量约为189Kd。纸层析与气相层析分析表明其为单一葡聚糖。经高碘酸氧化,Smith降解,甲基化,气相层析,气质联机分析,核磁共振(~1H-NMR,^(13)C-NMR)谱及红外光谱测定等,可确定PC-4的主链结构由β(1→3)糖苷键相连的葡萄糖构成,部份残基C_6上带有分支。约每5个糖残基有两个侧链,侧链仅为1个葡萄糖残基。 给ICR小鼠腹腔注射PC-4,对移植性肿瘤S-180有一定的抑制作用,抑瘤率为67％。离体条件下,PC-4与肿瘤细胞S-180共同培养,则未显示抑瘤作用。The water-soluble polysaccharide PC-4 was isolated and further purified from, residue of fruit bodies of Pleurotus citrinopileatus. The molecular weight of PC-4 was about 189Kd. Gel chromatography on Sepharose CL-4B reveals that PC-4 is homogeneous. The chemical structure of PC-4 was determined by periodate oxidation, Smith degration, methylation, G. C, G. C.-M. S., 1H-NMR, 13C-NMR, I. R, etc. The PC-4 molecular was composed of β(1→3) linked glucopyranose as a main chain and (1→6) branch. It was about every five glucosyl residues with two single Glc branches. It was found PC-4 has antitumor activity on the implanted Sarcoma 180 in mice, inhibition ratio was 67%; it was not found the antitumor activity on S-180 in vitro.

关 键 词：金顶侧耳 抗癌药 菌类药 药理学 

分 类 号：R282.710.5[医药卫生—中药学]