电针对内毒素休克大鼠肝脏的影响(组织化学观察)  

Histochemical Observation of the Effect of Electroacupuncture on the Livers of Rats with Endotoxic Shock

作　　者：黄为敏[1] 黄坤厚[1] 徐青燕[1] 王志英[1] 孙亚川 蔡虹[1] 张新宇[1] 林景蔚 

机构地区：[1]中国中医研究院针灸所,北京100700

出　　处：《针刺研究》1995年第3期36-39,共4页Acupuncture Research

基　　金：国家自然科学资金

摘　　要：本研究以组化法观察电针对内毒素休克鼠肝脏的影响。结果表明内毒素休克鼠肝糖元几乎全部脱失，葡萄糖－6－磷酸酶（G－6－Pase），琥珀酸脱氨酶（SDH），5’－核苷酸酶（5’－Nase），镁激活的三磷酸腺苷（Mg++－ATPase）活性减弱，电针后糖元含量部分增加，SDH、Mg＋＋－ATPase和G－6－Pase活性较休克对照组增加。提示电计“人中”或“足三里”穴可能对改善肝脏代谢和提高膜的转运起一定的作用。The expcrimental rats were randomly divided into three groups: L e. controI group; endotoxicshock group (the model of endotoxic shock was induced by intravenous administration of E. Coli endotoxin, 16mg/kg ): and electroacupuncture (EA ) group (' Renzhong' or ' Zusanli)' acupolnt wasstirnulated for 1o minutes at l hr after injection of endotoxin)- The experimental rats were decapitated at 75 minutes after injccting endotoxin. Their livers were taken out for cryostat section - and histo-chemical observation. The results were as follows: 1 ) The glycogen in the hepatic cells of endotoxicshocked rats were alrnost completely depleted- The activities of SDH Mg++u ATPase. G-6-Pase and5'~Nase were decreased: especially the activities of 5'-Nase 1n the biliary canallcull and sinusoids wereapparently rec1uced. 2 ) The content of hepatic glycogen in EA group was increased, but some of themwas still dePleted. The activities of SDH, Mg++-ATPase and G-6-Pase were slightly increased ascompared wlth that of the endotoxic shock group. The activity of 5'uNase was obviously increased afte r EA. The prelin1inary results indicated that EA at 'Renzhong' or IlZusanliI' acupoint of rats withndotoxic shock mlght play certain role on lmproving the hepatic metabolism and prornoting the rnembrane transport action

关 键 词：内毒素休克 电针"人中" 组织化学 肝脏 肝糖元 酶 

分 类 号：R245.97[医药卫生—针灸推拿学]