豌豆生殖细胞的发育──着重论述质体母系遗传的细胞学基础  

STUDIES OF GENERATIVE CELL DEVELOPMENT IN PISUM SATIVUM——WITH SPECIAL REFERENCE TO CYTOLOGI CAL BASIS OF PLASTID MATERNAL INHERITANCE

作　　者：刘兴梁[1] 胡适宜[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《Acta Botanica Sinica》1995年第10期749-753,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金;高等学校博士点专项科研基金

摘　　要：超微结构的研究证明，豌豆（Ｐｉｓｕｍ ｓａｔｉｖｕｍ Ｌ．）生殖细胞自形成直至成熟花粉时期，始终存在少量质体和较多的线粒体。ＤＮＡ 荧光的观察表明，在发育早期的生殖细胞中不含细胞质ＤＮＡ 类核，但在成熟花粉的生殖细胞中有许多的类核。在花粉离体萌发过程中，随着花粉管的生长，生殖细胞中的类核逐渐降解。在花粉培养２４ ｈ 后，生殖细胞的类核全部消失。研究结果确定了豌豆质体母系遗传的细胞学基础，支持遗传分析及ＲＦＬＰ研究的结论。It was proved by ultrastructural observations that few plastids and abundent mitochondria were ever present in the generative cell of Pisum sativum L. from its initiation to maturation. Fluorescence observations of DNA showed that many cytoplasmic DNA nucleoids were present in generative cell of mature pollen, but none in the early developing generative cell. During the germination of mature pollen in vitro, the cytoplasmic DNA nucleoids of the generative cell in the pollen tube degenerated gradually following the growth of the pollen tube and disappered completely 24 h after germination. The results provided a cytological basis for confirming the conclusion of plastid maternal inheritance in P. sativum obtained from genetic study, and resolved the contradiction between results from cytological observation and genetic or RFLP analysis.

关 键 词：豌豆 生殖细胞 质体遗传 发育 细胞学 

分 类 号：S643.303.2[农业科学—蔬菜学]