冷锻炼对水稻和黄瓜幼苗SOD、GR活性及GSH、AsA含量的影响  

EFFECT OF COLD HARDENING ON SOD AND GLUTATHION REDUCTASE ACTIVITIES AND ON THE CONTENTS OF THE REDUCED FORM OF GLUTATHION AND ASCORBIC ACID IN RICE AND CUCUMBER SEEDLINGS

作　　者：王以柔[1] 曾韶西[1] 刘鸿先[1] 

机构地区：[1]中国科学院华南植物研究所,广州510650

出　　处：《Acta Botanica Sinica》1995年第10期776-780,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：水稻（Ｏｒｙｚａ ｓａｔｉｖａ Ｌ．）和黄瓜（Ｃｕｃｕｍ ｉｓｍ ｅｌｏ Ｌ．）幼苗在昼夜温度为１５ ℃／１０ ℃、白天光照１２ ｈ，光强为２５０ μｍ ｏｌ·ｍ － ２·ｓ－ １的条件下锻炼３ ｄ，明显地提高幼苗叶片中膜保护酶——超氧物歧化酶（ＳＯＤ）、谷胱甘肽还原酶（ＧＲ）活性和内源抗氧化剂——还原型谷胱甘肽（ＧＳＨ）、抗坏血酸（ＡｓＡ）的含量。经冷锻炼和未锻炼的幼苗移置４ ℃、光强为２５０ μｍ ｏｌ·ｍ － ２·ｓ－ １下胁迫处理２ ｄ，未锻炼苗叶片中ＳＯＤ、ＧＲ 活性和ＧＳＨ、ＡｓＡ 含量明显下降，而经冷锻炼的苗则相对比较稳定。从脂质过氧化产物——丙二醛（ＭＤＡ）含量及幼苗的存活率亦看出：冷锻炼苗具有较低的脂质过氧化水平和较高的幼苗存活率。由此认为：冷锻炼能提高水稻和黄瓜幼苗细胞膜的稳定性。Rice and cucumber seedlings were employed in the study on changes of superoxide dismatase (SOD) and glutathione reductase (GR) activities, the contents of the reduced form of glutathione (GSH) and ascorbic acid (AsA) in leaves and the chilling resistance as well as the level of lipid peroxidation products after cold hardening and chilling stress under light. The seedlings hardened under a day/night temperature variation of 15 ℃/10 ℃ and photon flux density (PFD) of 250 μmol·m -2 ·s -1 for 12 h/d indicated an increase of the activities of SOD and GR, and the contents of GSH and AsA. The resistance of the seedlings to chilling and light stress was enhanced by cold hardening. Under the stress condition, the stabilities of SOD and GR activities, and contents of GSH and AsA in hardened seedlings were higher than those in the unhardened seedlings; the lipid peroxidation was also less than that in the latter. It was thus concluded that cold hardening under appropriate light leads on to the enhancement of function of membrane protective system and increase of cell membrane stability which is an important part of chilling resistance mechanism in the plant.

关 键 词：水稻 黄瓜 冷锻炼 超氧化物歧化酶 GR GSH ASA 

分 类 号：S511.101[农业科学—作物学] S642.201