MECHANISM OF CHANGE IN THE CATION INDUCED EXCITA TION ENERGY DISTRIBUTION BETWEEN PSⅡ AND PSⅠ IN THE CHLOROPLAST MEMBRANE FROM ZOSTERA MARINA *  

阳离子诱导大叶藻叶绿体膜中激发能在PSⅡ和PSⅠ之间分配变化的机理(英文)

作　　者：高振泮[1] 娄清香[1] 马红[2] 翟小京[2] 马桂芝[2] 毛大璋[2] 李良璧[2] 

机构地区：[1]中国科学院海洋研究所,青岛2660711 [2]中国科学院植物研究所,北京100044

出　　处：《Acta Botanica Sinica》1995年第11期833-841,共9页Acta Botanica Sinica（植物学报：英文版）

基　　金：the NationalNaturalScience Foundation ofChina and partly supported

摘　　要：The interrelations between thylakoid polypeptide components and Mg 2+ induced Chl a fluorescence and thylakoid surface charge changes were investigated in Zostera marina chloroplasts treated with Ca 2+ and trypsin. It was observed that:1. The increase of Mg 2+ induced PSⅡ fluorescence intensity was closely related to the decrease of Mg 2+ induced surface charge density of the thylakoid membrane in the normal chloroplast; 2. Removal of the 32~34 kD polypeptides of the thylakoid surface by Ca 2+ extraction of the chloroplast did not affect the Mg 2+ induced phenomena; 3. If the Ca 2+ treated chloroplast was further digested by trypsin to remove the 26 kD polypeptide of the membrane surface, the Mg 2+ induced phenomena disappeared completely. These results clearly indicated that the 26 kD polypeptide of thylakoid surface is the specific acting site of the cation that induced these two correlated phenomena in the chloroplast from Zostera marina. The mechanism on the regulating effect of the cation on excitation energy distribution between PSⅡ and PSⅠ was discussed.用Ｃａ２＋ 和胰酶处理大叶藻（Ｚｏｓｔｅｒａ ｍ ａｒｉｎａ）叶绿体膜研究了其类囊体膜多肽成分与Ｍｇ２＋ 诱导其Ｃｈｌａ荧光和类囊体膜表面电荷变化之间的相互关系，观察到：１．在正常的叶绿体膜中，Ｍｇ２＋ 诱导ＰＳⅡ荧光强度的增高与其诱导类囊体膜表面电荷密度的降低密切相关；２．用Ｃａ２＋ 处理这种叶绿体膜，除去类囊体膜表面的３２～３４ ｋＤ多肽对Ｍｇ２＋ 诱导的上述现象无影响；３．如果用胰酶消化Ｃａ２＋ 处理过的叶绿体膜，进一步除去膜表面的２６ ｋＤ多肽，Ｍｇ２＋诱导的这些现象则全部消失。这些实验结果清楚地表明，在大叶藻的叶绿体膜中，类囊体膜表面的２６ ｋＤ 多肽是阳离子诱导这两种相关现象的特异性作用部位。

关 键 词：CHLOROPLAST PHOTOSYSTEM Excitation energy distribution 

分 类 号：Q949.205[生物学—植物学]