Ca^(2+)、Mn^(2+)等8种金属离子对软腐欧文氏菌一些致病因子的影响  

EFFECT OF 8 METAL IONS ON PATHOGENIC FACTORS OF SOFT ROT ERWINIA

作　　者：张学君[1] 王金生[1] 方中达[1] 

机构地区：[1]南京农业大学植保系,南京210095

出　　处：《植物病理学报》1995年第1期79-84,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金

摘　　要：在离体试验中,不同金属离子对马铃薯软腐欧文氏菌(Erwinia carotovora subsp.carotovora)菌株StEcc-12生长,产生胞外酶和胞外酶活性具有不同的影响。在含果胶酸钠的培养液中,Ca^(2+)促进生长,并使培养液中的果胶裂解酶(PL)、果胶水解酶(PG)和蛋白酶3种胞外酶分别提高1.4～23.4倍,0.3～2.9倍和0.7～6.5倍。Mn^(2+)对StEcc-12生长及产生3种胞外酶的影响与Ca^(2+)相似。Ni^(2+)显著抑制细菌的生长,但在含Ni^(2+)培养液中3种胞外酶的单位活性比对照高1.7倍以上。在酶粗提液中分别加入上述3种离子对PL酶的活性均有抑制作用。Zn^(2+)对细菌生长没有显著影响,高浓度Al^(3+)抑制细菌生长,这2种离子均能促进3种胞外酶的产生。随着Fe^(2+)和K^+浓度的增高,细菌生长减少,两种果胶酶产量降低,蛋白酶产量增加,粗提液中PL酶活性下降。Mg^(2+)对细菌生长和胞外PG产生没有明显影响,提高Mg^(2+)浓度有利于PL的产生。酶粗提液中加入Mg^(2+)后PL活性增高。所有以上离子都能使聚半乳糖醛酸发生不同程度的凝聚,但只有Ca^(2+)和Mg^(2+)的有效凝聚浓度与薯块的实际浓度接近。Different metal ions had various effect on in vitro growth production of extracellular enzymes and enzyme activities of strain StEcc-12 of potato soft rot ( Erwinia carotovora subsp. carotovora ). In liquid media containing polygalacturonate, Ca2+ increased growth of StEcc-12 and raised its production of extracellular pectic lyase (PL), polygalacturonase (PG) and protease up to 1. 4~23. 5, 0. 3~2. 9 and 0. 7~6. 5 times, respectively. Mn2+ had the same effect as Ca2+ on bacterial growth and production of 3 extracellular enzymes. Ni2+ inhibited significantly the growth of pathogen, but the unit activities of 3 extracellular enzymes in suspension containing Ni2+ is 1. 7 times higher than check. Addition of above 3 ions alone into a crude extraction inhibited PL activities. Zn2+ han no effect on bacterial growth, and higher content of A13+ inhibited, but these 2 ions increased production of extracellular enzymes. As Fe2+ and K+ increased, bacterial growth and PL activities in extract solution decreased, synthesis of PL and PG reduced, but protease production raised. Mg2+ had no significant effect on in vitro growth and extracellular PG. Increased Mg2+promoted PL synthesis and its activities. All 8 ions could agglutate poly-galacturonic sodium, but only Ca2+ and Mg2+ effectively binding it at a content similar to those in potato tubers (102. 2ppm and 179. 2ppm, respectively).

关 键 词：软腐欧文氏菌 金属离子 致病因子 马铃薯 软腐病 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]