细菌凝集素与菌体脂多糖在大白菜与软腐欧氏杆菌接触识别中的作用  

THE NECESSARY FUNCTION OF AGGLUTININ AS COGNOR AND LIPOPOLYSACCHARIDE AS COGNON IN RECOGNITION BETWEEN CHINESE CABBAGE AND ERWINIA CAROTOVORA SUBSP. CAROTOVORA

作　　者：董汉松[1] 王金生[2] 方中达[2] 

机构地区：[1]山东农业大学植保系,泰安271018 [2]南京农业大学植保系,南京210095

出　　处：《植物病理学报》1995年第1期51-56,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(南京农业大学承担)

摘　　要：用3种试验方法测定了大白菜细菌凝集素(Agin-SD60)和软腐欧氏杆菌脂多精(LPS),在双方接触识别中的作用。在吸附抑制试验中,来自大白菜和马铃曾的Agin-SD60显示约98％的吸附抑制效应,另3种植物的Agin-SD60及大白菜外源凝集素(lectin)和细咆壁蛋白质(CWP)无明显作用;同时用不同种类的7个菌侏的LPS作测定,只有病菌的LPS吸附抑制作用明显(93.37％),其胞外多糖(EPS)也无作用。在菌体凝集试验中,也只有大白菜和马铃薯的Agin-SD60表现50％～100％的凝集活性。在琼脂双扩散试验中,寄主Agin-SD60可与病菌菌体及其LPS发生免疫沉淀。这些结果说明,Agin-SD60和菌体LPS在大白菜与软幅欧氏杆菌接触识别中分别作为植物识别子(cognor)和细菌识别子(cognon)起作用。The Agin-SD60, an agglutinin from chinese cabbage, and lipopolysaccharide (LPS) of Erwinia carotovora subsp. carotovora were tested for their roles in the contact recognition of the plant with the pathogen by 3 methods. In adsorption inhibition tests, the Agin-SD60 from 5 plant species including chinese cabbage, cell wall protein (CWP) and lectin from the host, and LPS and EPS (extracellular saccharide) from 7 species of bacteria, were used as inhibitors of adsorption of the pathogen on root surfaces of the host. The A-gin-SD60 from chinese cabbage and potato showed about 98% adsorption inhibition, and no inhibition was given by Agin-SD60 from other plants and by lectin and CWP of chinese cabbage. Of LPS and EPS from different bacteria, only LPS of Ecc and Eca ( E. carotovora subsp. atroseptica ) was able to inhibit adsorption at 93. 37% and 78. 6% respectively. In agglutination tests, the pathogen was agglutinated by Agin-SD60 from Chinese cabbage and potato but other plants. Then the ODD tests were made with combinations of Agin-SD60 from different plants with LPS from different bacteria and EPS from the pathogen. Cross reactions were detected between LPS from Ecc and Agin-SD60 from chinese cabbage, and between Agin-SD60 from chinese cabbage and LPS from Ecc and Eca. These results indicated that Agin-SD60 of chinese cabbage is the cognor and LPS of the pathogen is the cognon, for the recognition between chinese cabbage and soft rot Er-winia.

关 键 词：大白菜 软腐病菌 凝集素 脂多糖 接触识别 

分 类 号：S436.341.1[农业科学—农业昆虫与害虫防治]