大麦黄花叶病的分子生物学与基因工程研究——Ⅱ.大麦黄花叶病毒抗性突破株系的分子分析  

MOLECULAR BIOLOGY AND GENETIC ENGINEERING OF BARLEY YELLOW MOSAIC DISEASE I . MOLECULAR ANALYSIS OF A RESISTANCE-BREAKING STRAIN OF BARLEY YELLOW MOSAIC VIRUS

作　　者：朱睦元[1] R. Stratford I. Fould 

机构地区：[1]杭州大学生物科学与技术系,杭州310012 [2]英国剑桥P.B.I.生物技术系

出　　处：《植物病理学报》1995年第2期117-123,共7页Acta Phytopathologica Sinica

基　　金：英皇家学会王宽诚教育基金会;国家自然科学基金;浙江省科委;教委基金;自然科学基金

摘　　要：大麦山黄花叶病毒抗性突破株系的出现克服了已有大麦抗性基因的作用。本研究通过对大麦黄花叶病毒原株系和抗性突破株系RNA1中包含NIa蛋白酶基因的1Kb区段进行克隆和序列分析,比较两株系的序列差异。表明两者间核苷酸水平的同源性为97.2％,氨基酸水平的同源性为97.3％。根据存在的这些微小差异,人工合成具有株系特异性的聚合酶链式反应(PCR)引物,建立PCR检测系统,以期在大田黄花叶病毒株系鉴定中应用。Barley yellow mosaic virus (BaYMV) causes one of the most important diseases of winter barley in parts of Europe and Asia and yield losses can only be prevented by cultivating resistant varieties. However, a resistance-breaking strain of BaYMV (race 2) has. recently been reported which overcomes the effect of original resistant gene. We have cloned and sequenced a 1 kb region from the putative NIa proteinase gene of a BaYMV race 1 and race 2 isolate. The two sequences displayed 97. 0% identity at nucleotide level and 97. 3% amino acid identity. This minor sequence variation was used to develop a PCR-based system using specific primers to distinguish these isolates.

关 键 词：大麦 黄花叶病毒 基因克隆 聚合酶链式反应 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治]