脱氧雪腐镰刀菌烯醇对K~＋刺激的小麦根质膜ATP酶活性、K~＋吸收、外渗及再分配的影响  

Effects of Deoxynivalenol on Activity of K￣+-Stheulated Root PM ATPase, and Uptake, Leakage and Allocation of K￣+ in wheat Seedlings

作　　者：姚泉洪[1] 黄晓敏[1] 刘宗镇[1] 康尧[1] 

机构地区：[1]上海市农业科学院

出　　处：《植物生理学报（0257-4829)》1995年第1期29-34,共6页Acta Phytophysiologica Sinica

摘　　要：１０－８ｍｏｌ／Ｌ的ＤＯＮ毒素加入小麦根质膜制剂中可促进Ｋ＋刺激的ＡＴＰ酶活力，１０－６ｍｏｌ／Ｌ开始呈抑制效应，抑制程度随ＤＯＮ浓度加大而提高。根尖（５ｃｍ）离体根段于０．５ｍｍｏｌ／Ｌ的ＫＣｌ中，１０－８ｍｏｌ／Ｌ的ＤＯＮ能促进根段Ｋ＋吸收，１０－６ｍｏｌ／Ｌ以上浓度则Ｋ＋吸收呈抑制，１０－２ｍｏｌ／Ｌ浓度下根段的净吸收为负值，表明组织中Ｋ＋大量外渗。根段置蒸馏水中６ｈ，４ｍｍｏｌ／Ｌ的ＤＯＮ即导致振段Ｋ＋渗漏。用ＤＯＮ处理整株小麦根，浓度在０．２５ｍｍｏｌ／Ｌ以上可促进Ｋ＋从植株其它部位向根运输，而浓度在８ｍｍｏｌ／Ｌ时即抑制Ｋ＋向根富集，且根内Ｋ＋明显渗漏。When Fusarium toxin deoxynivalenol (DON ) was added to a concentration of 10-s mol/L into plasmalemma-enriched preperation of wheatroot, the K+-stimulated ATPase activity was increased. However, when theDON concentrstion was raids to 10-6mol/L, inhibition occurred and its extent increased with the increase ofDON concentration. The K+ uptake of5 cm Segments from from root tip was enhanced by 10-8 mol/L DON, but lowered by a DON concentration higherthan 10-e mol/L. The net uptake wasnegative in 10-2 mol/L DON, implying that K+ was leaked from wheatroot. K+ leakage was observed inwheat root segment treated with 4mmol/L DON. 0. 25￣ 6. 0 mmol/LDON could stimulate K+ allocationfrom other perts of wheat plant to thepet treated with DON. The K+ accumulation was inhibited in & mmol/LDON and rod suffered a net loss ofK+.

关 键 词：脱氧雪腐镰刀 烯醇 小麦 钾 腺苷三磷酸酶 

分 类 号：S512.101[农业科学—作物学]