表油菜素内酯对cAMP在植物体内分在和对质膜ATPase活性的影响  

Effect of Epibrassinonde on the Distribution of cAMP and Activity of Plasma Membrane ATPase in Plant Tissue

作　　者：徐如涓 何宇炯[1] 吴登如 赵毓橘[1] 

机构地区：[1]中国科学院上海植物生理研究所

出　　处：《植物生理学报（0257-4829)》1995年第2期143-148,共6页Acta Phytophysiologica Sinica

基　　金：国家自然科学基金

摘　　要：植物材料经ｅｐｉＢＲ处理后，ｃＡＭＰ的水平明显提高，比对照增加１～３倍，其变化的时间进程有６ｈ的滞后期。小麦根系质膜ＡＴＰａｓｅ与ｅｎｉＢＲ一起温育，质膜ＡＴＰａｓｅ活性未表现提高，反而明显下降；当ｅｐｉＢＲ浓度提高到２×１０－６ｍｏｌ／Ｌ时，质膜ＡＴＰａｓｅ活性降低过半。在此反应系统中加入５×１０－５ｍｏｌ／Ｌ的ＩＡＡ后，质膜ＡＴＰａｓｅ活性明显提高，并超过了单独使用ＩＡＡ处理的材料。放射自显影表明，油菜素甾酮主要分布在绿豆幼苗上胚轴近真对端１ｃｍ处及黄瓜幼苗生长锥和子叶基部，均为形态生长旺盛的部位。The level of cAMP in cucumberhypocotyls was markedly enhanced byepiBR treatment (Fig. 2), and ranparallel to that of hypocotyl elongation, both showing a 6 h lag period(Fig. I). When the membranes ofwheat rootS were incubated with epiBR, the activity of ATPase of themembranes showed no increase, andeven decreased when epiBR concentration was at 2×X10-6 mol/L. But withIAA (5 ×10-5 mol/L) in the reactionsystem, the activity of ATPase waspromoted and was higher than thattreated with IAA alone (Fis. 5). Theradiograph revealed that 125 I-btassinos-terone was distributed mainly in the elongation zone of the epicotyls of mungbeau seedlings, but in the apex andcotyledon bases of cucumber seedlingS.Both were actively growing zones(Fig. 6).

关 键 词：表油菜素内酯 质膜 腺苷三磷酸酶 植物 环境腺苷 

分 类 号：S311[农业科学—作物栽培与耕作技术] S143.89[农业科学—农艺学]