荔枝和龙眼种子发育过程中ABA含量及对外源ABA敏感性的变化  

ABA Contents and Sensitivity to Exogenous ABA of Lychee (Litchi chinensis Sonn) and Longan (Euphoria loadan Steud) Seeds during Development

作　　者：彭业芳[1] 傅家瑞[1] 

机构地区：[1]中山大学生物系

出　　处：《植物生理学报（0257-4829)》1995年第2期159-165,共7页Acta Phytophysiologica Sinica

基　　金：国家自然科学基金

摘　　要：在荔枝和龙眼种子发育过程中，内源ＡＢＡ水平先是上升，至大约７８～８０ＤＰＡ时出现高峰，之后两者ＡＢＡ含量均不断下降。果实成熟时采收的种子，ＡＢＡ含量比高峰时分别下降近６倍。另外，随着种子的发育，种子及其胚轴对外源ＡＢＡ的敏感性（ＳＡＢＡ）亦持续下降。１０－４ｍｏｌ／ＬＡＢＡ可以完全抑制９０ＤＰＡ前的荔枝和龙眼种子的萌发，但对成熟种子１０－２ｍｏｌ／ＬＡＢＡ亦不能抑制其萌发。龙眼种子离体胚轴的ＳＡＢＡ高于荔枝。ＡＢＡ含量与敏感性的这种变化可能是两种顽拗性种子成熟时萌动，进而不耐脱水贮藏的重要原因之一。ABA contents, measured by enzyme linked immunosorbent assay(ELISA), increased obviously duringearly developttiental stagal of lycheeand longan seeds (Fig. 1). When itPeaked at 78 ￣ 80 days pet anthesis(DPA), ABA content was 986 ng/gDW in lychee seeds, and 1128 ng/gDW in longan seeds, then declinedmarkedly in both species. At harvest,ABA content of lychee and longanseeds was 144, 169 ng/g DW, respectively. Moreover, the sensitivity toABA (SABA ) of seeds (Fig. 2 ) andtheir isolated embryonic axes (Fig. 3)decreased during development. 10-4mol/L ABA could completely inhibitthe germination of lychee and longanseeds before 90 DPA; however, even10-2 mol/L ABA failed to completelyinhibit the germination of seeds harvested at full maturity. For young embryonic axes, 10-7 mol/L ABA inhib-ited their germination incubated onwoody plant medium (WPM), wheras10-4 mol/L ABA could hardly inhibitthe germination of embryonic axes iso-lated from mature seeds. The SABA oflongan embryonic axes was higher thanthat of lychee ones. The ABA contentand sensitivity in relation to seed reCalcitrance was discussed.

关 键 词：荔枝 龙眼 种子发育 顽拗性种子 脱落酸 

分 类 号：S667.01[农业科学—果树学] S330.29[农业科学—园艺学]