毛花猕猴桃原生质体再生植株  

PLANTLET REGENERATION FROM LEAF PROTOPLASTS IN SEEDLING OF ACTINIDIA ERIANTHA BENTH.

作　　者：张远记[1] 母锡金[1] 蔡起贵[1] 周云罗 钱迎倩[1] 

机构地区：[1]中国科学院植物研究所,北京100044

出　　处：《Acta Botanica Sinica》1995年第1期48-52,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家"八五"细胞工程资助项目

摘　　要：从毛花猕猴桃（Ａｃｔｉｎｉｄｉａ ｅｒｉａｎｔｈａ Ｂｅｎｔｈ．）试管培养的实生苗新展开叶片分离的原生质体，培养在液体ＭＳ（除去ＮＨ４ＮＯ３）附加２，４－Ｄ １．０ ｍ ｇ／Ｌ和葡萄糖０．４ ｍ ｏｌ／Ｌ的培养基上。培养３周后植板率达到１９．４％ 。在未添加新鲜培养基的情况下，原生质体再生的细胞可持续分裂，并于３个月时长成２ ｍ ｍ 大小的愈伤组织。将该愈伤组织转移到附加玉米素０．５ ｍ ｇ／Ｌ和ＩＡＡ ０．１ ｍ ｇ／Ｌ的固体ＭＳ培养基上，分化出苗。试管苗经诱导生根。Newly extended leaves of in vitro seedling of Actinidia eriantha Benth. were used for protoplast isolation.Protoplasts were cultured in liquid MS medium (devoid of NH 4NO 3) supplemented with 1 0 mg/L 2,4 D and 0 4 mol/L glucose.The plating efficiency after 3 weeks of culture was about 19 4%.Protoplasts derived cells divided sustainably and developed into calli of 2 mm in size in the original protoplast culture medium without adding fresh medium so to decrease the osmotic pressure.These calli regenerated shoots when being transfered to MS medium with 0 5 mg/L zeatin and 0 1 mg/L IAA.Regenerated shoots were rooted by immersion in 20 ppm IBA solution before culturing on half strength MS medium devoid of growth regulators.

关 键 词：猕猴桃 毛花猕猴桃 叶片 原生质体 植株再生 

分 类 号：S663.901[农业科学—果树学]