菜豆热激蛋白在生物膜上的定位  

LOCALIZATION OF HEAT SHOCK PROTEIN ON CELL MEMBRANES OF PHASEOLUS VULGARIS

作　　者：刘箭[1] 杨晓贺[1] 吴显荣[1] 

机构地区：[1]北京农业大学,北京100094

出　　处：《Acta Botanica Sinica》1995年第2期87-90,共4页Acta Botanica Sinica（植物学报：英文版）

摘　　要：选用菜豆 Phaseolus vulgris L. 下胚轴 ,运用35S- Met标记放射自显影和二维电泳技术 ,研究热激蛋白 HSPs 的表达和在生物膜组分中的定位 .实验结果表明 ,盐溶蛋白中主要HSPs为 70 k D HSPs和小分子量 HSPs,而小分子量组 HSPs大量富集在质膜和液泡膜组分中 .Experiment was designed to investigate the expression of heat shock proteins (HSPs) in hypocotyls of Phaseolus vulgaris L.and HSPs localization on cell membranes by means of 35 S Met labelling, fluorography of SDS PAGE and 2 D electrophoresis.The results showed that 70 kD group HSPs were the major labelled proteins and the small HSPs accumulate largely in the fractions of plasma membrane and tonoplast membrane.

关 键 词：热激蛋白 质膜 液泡膜 菜豆 生物膜 定位 

分 类 号：S643.101[农业科学—蔬菜学] Q949.751.9[农业科学—园艺学]