羊草抗羟脯氨酸细胞变异系的筛选及其特性分析  

ISOLATION AND CHARACTERIZATION OF A CHINESE ANEUROLEPIDIUM CELL LINE RESISTANT TO HYDROXYPROLINE

作　　者：陈晖[1] 匡柏健[1] 王敬驹[1] 

机构地区：[1]中国科学院植物研究所,北京100044

出　　处：《Acta Botanica Sinica》1995年第2期103-108,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：离体培养条件下 ,用脯氨酸类似物羟脯氨酸 ( HYP) 2 0 mmol/L作选择压力 ,筛选出羊草( Aneurolepidium chinense( Trin.) Kitag.)抗 HYP细胞变异系 HR2 0 - 8。其游离脯氨酸含量比供体提高 6.6倍 ,而且抗 Na Cl、PEG及低温的能力也较供体增强。对变异系脯氨酸合成途径进行分析 ,发现其中控制第一步反应的关键酶—— -谷氨酸激酶的活性异常增加 ,比供体高 2 .5倍。而且外源脯氨酸对变异系和供体的 -谷氨酸激酶的抑制表现得很相似。A hydroxyproline(HYP) resistant cell line of Chinese aneurolepidium(Aneurolepidium chinense (Trin.) Kitag.) was isolated under selection pressure of 20 mmol/L HYP. Comparison of the free amino acid pool levels in the cell line with that of donor showed substantial accumulation of proline(6 6×).Enzyme examination indicated that  glutamyl kinase controlling proline biosynthesis in HR20 8 cell line had 2 5 times as much activity as that of the donor. Exogenous L proline inhibited the enzyme activities both in the HR20 8 and the donor by the same rate of 30% at 100 mmol/L. The responses of HR20 8 cell line to NaCl,PEG and cold temperature (5℃) were also compared with those of donor and the former exhibited remarkably increased tolerance to the tested stress condition. The results showed that changes of  glutamyl kinase property exhibited the phenomenon of extra accumulation of proline which might favor the increased tolerance to NaCl,PEG and cold temperature in the resistant cell line.

关 键 词：羊草 变异系 组织培养 脯氨酸 

分 类 号：S543.903.5[农业科学—作物学] Q949.714.2[生物学—植物学]