光系统Ⅱ反应中心D1/D2/Cytb559复合物中存在一个与光化学活性无关的去镁叶绿素a  被引量:6

OBSERVATION OF ONE PHEOPHYTIN A MOLECULE NOT ASSOCIATED WITH PRIMARY PHOTOCHEMISTRY IN THE ISOLATED PHOTOSYSTEM Ⅱ REACTION CENTER D1/D2/CYT B559 COMPLEX

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作  者:侯建敏[1] 彭德川[1] 匡廷云[1] 唐崇钦[1] 汤佩松[1] 

机构地区:[1]中国科学院植物研究所光合作用研究室,北京100044

出  处:《Acta Botanica Sinica》1995年第5期405-408,共4页Acta Botanica Sinica(植物学报:英文版)

基  金:国家自然科学基金;中国科学院重点科研项目

摘  要:Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The results showed that: (1) There is one pheo a molecule which is not associated with the primary photochemistry in the PSⅡ reaction center complex. It may be considered that there are two different electron transfer branches in the PSⅡ reaction center just as in the purple bacterium photosynthetic reaction center. (2) The damaged pheo a may be attributed to the one bonding to the D2 protein comparing the D2 subunit in the PSⅡ reaction center with M subunit in the purple bacterium photosynthetic reaction center. (3) A possible arrangement model of redox cofactors in the PSⅡ reaction center was proposed based on our experiment.Photodamage of pheophytin a (pheo a) in the isolated photosystem Ⅱ (PSⅡ) reaction center D1/D2/Cyt b559 complex from spinach has been investigated by high performance liquid chromatographic method in detail. The results showed that: (1) There is one pheo a molecule which is not associated with the primary photochemistry in the PSⅡ reaction center complex. It may be considered that there are two different electron transfer branches in the PSⅡ reaction center just as in the purple bacterium photosynthetic reaction center. (2) The damaged pheo a may be attributed to the one bonding to the D2 protein comparing the D2 subunit in the PSⅡ reaction center with M subunit in the purple bacterium photosynthetic reaction center. (3) A possible arrangement model of redox cofactors in the PSⅡ reaction center was proposed based on our experiment.

关 键 词:光合系统Ⅱ 反应中心 去镁叶绿素a 光破坏 

分 类 号:Q945.11[生物学—植物学] Q947.8

 

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