授粉诱导兰花花部乙烯生物合成基因在转录水平上的表达  

POLLINATION INDUCED EXPRESSION OF ETHYLENE BIOSYNTHETIC GENES AT TRANSCRIPTION LEVEL IN ORCHID FLOWERS

作　　者：张宪省[1] 闫先喜[1] 马小杰[1] 

机构地区：[1]山东农业大学,泰安271018

出　　处：《Acta Botanica Sinica》1995年第5期356-360,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：朵丽蝶兰（Ｄｏｒｉｔａｅｎｏｐｓｉｓｈｙｂｒｉｄａ Ｈｏｒｔ．）的花授粉后，测定乙烯的产生，并分析授粉后花部各器官乙烯生物合成的ＡＣＣ合成酶和ＡＣＣ氧化酶两个基因转录水平上的表达。授粉后在花部均可探测到ＡＣＣ合成酶和ＡＣＣ氧化酶的ｍ ＲＮＡ。在花部不同器官之间，此两种酶的ｍ ＲＮＡ的积累水平均表现出一些差异。ＡＣＣ合成酶的ｍ ＲＮＡ 积累与ＡＣＣ氧化酶相比，具有更明显的特异性。而ＡＣＣ氧化酶ｍ ＲＮＡThe authors investigated pollination induced ethylene production and expression patterns of genes encoding 1 aminocyclopropane 1 carboxylate (ACC) synthase and ACC oxidase in orchid flowers (Doritaenopsis hybrida Hort.). Following pollination both ACC synthase and ACC oxidase mRNAs were detected in the different organs of flowers, and the patterns of both ACC synthase and ACC oxidase mRNA accumulation were similar. mRNA accumulation of ACC synthase mRNA was more organ specific than that of ACC oxidase mRNA. However, ACC oxidase mRNAs were much more abundant than ACC synthase mRNAs in the flower organs.

关 键 词：授粉 乙烯 基因转录 朵丽蝶兰 兰花 生物合成 

分 类 号：S682.310.3[农业科学—观赏园艺] Q949.718.4[农业科学—园艺学]