在水稻花培后代中RFLP零等位的遗传分析  

GENETIC ANALYSIS OF NULL ALLELES IN RICE DOUBLED HAPLOID PLANTS FROM ANTHER CULTURE

作　　者：李平[1] 朱立煌[1] 陈英[1] 陆朝福 周开达[2] 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]四川农业大学水稻研究所,温江611130

出　　处：《Acta Botanica Sinica》1995年第5期351-355,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：在构建水稻分子图谱的研究中，有４ 个ＲＦＬＰ探针揭示了一对籼粳稻之间的零等位现象．其中ＲＧ ２２９ 揭示了籼稻“圭６３０”（Ｏｒｙｚａ ｓａｔｉｖａ ｓｓｐ． ｉｎｄｉｃａ）两个零等位位点；ＲＧ ４１９，ＲＧ４２４ 及ＲＧ ３５３ 揭示了粳稻“０２４２８”（Ｏ． ｓａｔｉｖａ ｓｓｐ．ｊａｐｏｎｉｃａ）的零等位． 利用８１ 个圭６３０／０２４２８ 的花培植株（ＤＨ）进行的遗传分析结果表明，这些零等位位点与ＭｃＣｏｕｃｈ 发表的水稻分子图谱中其相邻分子标记间的连锁关系发生了变化．另外，用ＲＧ ６８４ 探针在一些ＤＨ植株中检测出有新的零等位出现，虽然双亲都具有与ＲＧ ６８４ 同源的序列．In the research of constructing a rice (Oryza sativa) molecular map, 4 RFLP markers, i.e. RG 229, RG 419, RG 424 and RG 353, detected the null alleles in the indica and japonica parental rice.RG 229 indicated two null alleles in indica rice Gui 630, and each of the other markers revealed a null allele in japonica rice 02428. Genetic analysis in the doubled haploid (DH) population consisting of 81 plants showed that the linkage relationships between these null allele loci and the neighboring molecular markers shown in McCouch’s rice molecular map were changed. In addition, the marker RG 684 could detect its null alleles in some DH plants, though the RG 684 sequence did exist in the genomes of both parents. Appearence of null alleles might be induced by transpositional changes on chromosomes.

关 键 词：水稻 RFLP分析 零等位基因 花药培养 遗传分析 

分 类 号：S511.103.2[农业科学—作物学]