小分子量热激蛋白胶体金免疫电镜定位  

LOCALIZATION OF HEAT SHOCK PROTEIN OF LOW MOLECULAR WEIGHT BY ELECTROMICROSCOPIC IMMUNOGOLD LABELLING

作　　者：刘箭[1] 杨晓贺[1] 吴显荣[1] 

机构地区：[1]北京农业大学生物学院,北京100094

出　　处：《Acta Botanica Sinica》1995年第8期652-654,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：高校博士学科点专项科研基金资助

摘　　要：Inspite of the large amount of low molecular weight heat shock protein (LMW HSP) present in plant, its function has still not been clearly known. Understanding the distribution and movement of LMW HSP in cells could provide useful information about its biological functions. A 14 kD HSP was purified from the microsome isolated from the bean of a highly thermotolerant plant Phaseolus vulgaris. Antiserum against this protein was preparaed. The localization of the protein in the cell was analysed by means of electromicroscopic immunogold labelling method. The images of electromicrograph showed that 14 kD HSP mainly existed in both cytoplasm and endoplasmic reticulum and that no labelled gold was found in tonoplasma, mitochondria or cell wall.Inspite of the large amount of low molecular weight heat shock protein (LMW HSP) present in plant, its function has still not been clearly known. Understanding the distribution and movement of LMW HSP in cells could provide useful information about its biological functions. A 14 kD HSP was purified from the microsome isolated from the bean of a highly thermotolerant plant Phaseolus vulgaris. Antiserum against this protein was preparaed. The localization of the protein in the cell was analysed by means of electromicroscopic immunogold labelling method. The images of electromicrograph showed that 14 kD HSP mainly existed in both cytoplasm and endoplasmic reticulum and that no labelled gold was found in tonoplasma, mitochondria or cell wall.

关 键 词：植物 小分子量 热激蛋白 胶体金免疫电镜 菜豆 

分 类 号：Q946.1[生物学—植物学] S643.101[农业科学—蔬菜学]