应用酯酶同工酶对甘草属长荚果系的不同种亲缘关系的研究  

A STUDY ON RELATIONSHIP OF DIFFERENT SPECIES OF GLYCYRRHIZA LONGILEGUMATA THROUGY ESTERASE-ISOZYMES

作　　者：潘鼎元[1] 张煜星[1] 李学禹[1] 

机构地区：[1]新疆石河子农学院

出　　处：《植物研究》1995年第4期477-484,共8页Bulletin of Botanical Research

摘　　要：对甘草属的长荚果系，采取聚丙稀酰胺凝胶电泳法，分析它们的酯酶同工酶。结果表明：每个种具有明显的酶谱差异，有其特征酶带；从酶谱的相似度指数来看，６．６ａ、８、８ａ的相似度指数较高，亲缘关系较近。而２、４、５、８ｂ的相似性度指数较低，它们之间的亲缘关系较远。经过簿层色谱扫描，根据各酶谱的峰面积的百分率，用系统聚类法，最短距离法进行聚类分析，以欧氏距离４．０为分类线，把甘草属长荚果系分为二类，Ⅰ类：１、５、６、６ａ、７、８、８ｂ；Ⅱ类２ａ、４、８ａ、３、２．The Esteras-isozymes of Glycyrrhiiza Longilegumata X. Y. Li have beencomparatively studied by means of polyarcylamide gel electrophoresis. The interspecific zymogramatic differences are obvious. Each species possesses its specificzymograms, distinguishable from the others. By means of the zymogramatic indexof similarity these species was analysed. It shows the close affinity among 6. 6a.8. 8a there is high index of similarity, but they are very far from 2, 4, 5, 8b onthe basis of percentage of each zymogramatic peak area with the scanning chromatograms. By means of the systematic cluster and the short distance were clusteranalysis. When the Euclid's distance was higher than 4. 0 or lower than 4. 0Glycyrrhiza Longilegumata X. Y. Li may be grouped in two categories. 1,5,6,6a,7,8,8b into onecategory, the seconed category conzains 2a,4,7a,3,2.

关 键 词：甘草属 长夹果系 种 酯酶同功酶 

分 类 号：Q949.751.9[生物学—植物学]