(15)~N_2还原法测定胡萝卜离体类根瘤的固氮作用  

NITROGEN FIXATION OF PARA-NODULES OF ORGAN CULTURED CARROT ROOTS WITH ^(15)N_2 REDUCTION METHOD

作　　者：吴观以[1] 李慧荃[1] 李元芳[1] 孙建光[1] 姚允寅[2] 陈明[2] 张希忠[2] 

机构地区：[1]中国农业科学院土壤肥料研究所,100081 [2]中国农业科学院原子能利用所,100094

出　　处：《植物营养与肥料学报》1995年第2期61-64,共4页Journal of Plant Nutrition and Fertilizers

基　　金：总理基金

摘　　要：用低浓度的2,4-D处理胡萝卜离体根诱导结瘤,并接种固氮螺菌(Azospirillum brasilense),用^(15)N_2还原法测定结果证明,胡萝卜离体类根瘤能固定^(15)N_2。电镜切片观察发现,类根瘤内有菌体存在,菌体进入细胞间隙中比进入细胞内多。Nitrogen fixation of para-nodules of organ cultured carrot roots was assured with 15N2 reduction method after the par a-nodules of carrot roots were induced with low concentration 2,4-D and the inoculation of Azospirillum brasilense. More bacteria in intercellular spaces and less in cells of para-nodules were also observed under electronic microscope.

关 键 词：胡萝卜 离体根 类根瘤 ^15N2还原法 固氮作用 

分 类 号：S631.201[农业科学—蔬菜学]