镰刀菌培养物中代谢产物的GC／MS鉴定与定量分析  

Identification and Quantitation of Fusarium Metabolites in Rice Cultures of Fusarium Fungi by GC/MS

作　　者：罗毅[1] 刘锋[1] 冯建林[1] 胡绪英[1] 杨进生[1] 匡开源[1] 史士英[1] 

机构地区：[1]北京军事医学科学院毒物药物研究所,上海农业科学院植物保护研究所

出　　处：《质谱学报》1995年第4期54-58,共5页Journal of Chinese Mass Spectrometry Society

摘　　要：本文报道对镰状镰刀菌菌株（ＦｕｓａｒｉｕｍｆｕｓａｒｉｏｉｄｅｓＦｒａｇ．＆Ｃｉｆ．）产毒培养物中的有毒代谢产物用ＧＣ／ＭＳ鉴定和单离子选择定量分析。结果表明镰状镰刀菌菌株产毒培养物中产生的主要是Ｔ－２毒素组的单端孢霉烯族毒素，这对探讨产毒菌株和产生的有毒代谢产物是很有意义的。In this paper,it is mainly reported on results of identification and quantitation of toxic metabolites in rice culture of Fusarium fusarioides(Frag. & Cif. )by GC/MS/El+,which was isolated from the ear of red scab wheat. Rice culture of Fusarium fusarioides(Frag. &Cif) was extracted with acetonitrile: water (3:1, V/V)followed by defatted with n-he-cane. The extract was cleaned up on the column of Florisil and eluted with chloroform: methane(9:1,V/V). The eluate was concentrated to dryness underreduced pressure. The residue was derivatizrf with heptafluorobutyrylimidazole(HFBIM) followed by GC/MS analysis. By the identification of GC/MS,it was found that in the culture extract Fusarium fusarioides(Frag. & Cif. ) produced the secondary toxic metabolites, such as T-2 tetraol, T-2 triol, 3'-hydroxyl T-2 toxin, neosolaniol(NS),HT-2 toxin and T-2 toxin etc. The contents of these produced toxins were quantitatively determined with selected single ion, respectively,compared with standard of each toxin.It was confirmed that the strain of Fusarium fusarioides(Frag. & Cif. )mainly produced those trichothecenes belonged to T-2 toxin group. The results are of significant for the study on Fusarium fungi and their toxic metabolites.

关 键 词：镰刀菌 单端孢霉烯族 产毒菌株 GC/MS 

分 类 号：Q939.5[生物学—微生物学] O657.6[理学—分析化学]