松果有效成分的研究Ⅳ.红松果多糖中酸性糖的含量测定  

Studies on Effective Composition of Pinecone Ⅳ.Determination of Acid Polysaccharides in Polysaccharides of Cone of Korean Pine (Pinus koraiensis)

作　　者：李好枝[1] 吕永俊[1] 梁卉[1] 郭铁军 

机构地区：[1]沈阳药科大学 [2]大庆市第三人民医院

出　　处：《中草药》1995年第7期347-348,350,共3页Chinese Traditional and Herbal Drugs

摘　　要：用硫酸-咔唑比色法测定了红松果多糖中酸性糖的含量，并考察了浓硫酸与咔唑的用量及反应温度、时间等因素对显色反应的影响，最大吸收波长520nm，测得酸性糖在1．0～7．0μg／ml浓度范围内与吸收度呈线性关系（r＝0．9999，n＝7），平均回收率为99．0％～99．8％，RSD为1．3％～2．3％（n＝6），最低检测浓度为0．05μg／ml。Acid polysaccharides in pinecone were determined quantitatively by sulfuric acid-carba zole colorimetry and the influence of amount of sulfuric acid carbazole, reaction temper ature and time forcolor-reaction were investigated. The maximum abstrption was found to be at 520nm. The absorbance was a linear function of concentrations between 0 and 7 μg/ml acid polysaccharides (r = 0.9999, n = 7). Analytical recovcry was 91.0%～99.8% . RDS was 1.3%～2.3% (n = 6 ). The minimum detectable concentration was 0.05μg/ml.

关 键 词：红松果多糖 硫酸-咔唑法 松果 红松 酸性糖 

分 类 号：R284.2[医药卫生—中药学]