六种中草药有效成分对钙调素依赖环核苷酸磷酸二酯酶的作用  

Effects of Six Effective Components of Chinese Herbal Drugs on Calmodulin-Dependent Cyclic Nucleotide phosphodiesterase

作　　者：杨东丽[1] 陈鋆[1] 顾熊飞[2] 马涧泉[2] 聂磊[2] 任晖[2] 

机构地区：[1]皖南医学院 [2]中山医科大学

出　　处：《中草药》1995年第11期582-584,共3页Chinese Traditional and Herbal Drugs

基　　金：美国中华医学会(CMB)基金

摘　　要：用正交设计观察了6种中草药有效成分和三氟啦嗪（TFP）对钙调素依赖环核苷酸磷酸二酯酶（CaM-PDE）激活活性的影响，结果表明：a）两面针碱（Nit）、槲皮素（Que）和TFP对CaM－PDE激活活性具有明显抑制作用（P＜0．01），其抑制强度：Qne＞TFP＞Nit；小檗碱（Ber）、麻黄碱（Eph）和鱼腥草素（Hou）对CaM－PDE无明显作用（P＞0．05），青藤碱（Sin）对酶的作用可疑；c）Qne与TFP、Nit与TFP之间有协同作用。结果提示Nit和Que可能是CaM拮抗剂，两者的作用位点似乎不同于TFP。Effects of nitidine (Nit ), berberine (Ber ), sinomenine (Sin ), ephedrine (Eph ), houttuynin (Hou ), quercetin (Que ) and trifluoprazine (TFP ) on calmodulin-dependent cYclic nucleotide phosphodiesterase (CaM-PDE ) were investigatcd by orthogonal design.Our observation came out that Nit, Que and TFP significantly inhibited the CaM-activated PDE activity (P<0.01 ) in ,the order of Que>TFP>Nit. Ber, Eph, and Hou had no effects on the enzyme activity (P>0.05 ). The effect of Sin on CaM-PDE was uncertain.It was also shown that there were cooperativity between Nit and TFP and synergism between Que and TFP. These results suggested that Nit and que may act as calmodulin antagonists and might have. different sites on calmodulin in comparison with those for TFP.

关 键 词：环核甙酸 磷酸二酯酶 钙调素 两面针碱 中草药 

分 类 号：R285.5[医药卫生—中药学]