乙型脑炎病毒寡核苷酸探针的化学合成及初步检测  

Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV

作　　者：项秉懿[1] 杨勇峰[1] 刘利兵[1] 马文煜[1] 姜绍谆[1] 苏成芝[1] 

机构地区：[1]第四军医大学分子生物学研究所

出　　处：《中国病毒学》1995年第2期131-134,共4页Virologica Sinica

摘　　要：根据已发表的乙型脑炎病毒核苷酸序列，设计合成了１９寡核苷酸，作为检测该病毒的基因探针。以大肠杆菌ＤＮＡ聚合酶ＩＫｌｅｎｏｗ片段标记合成的寡核苷酸后，经过分子杂交证明该探针能检测出１ｐｇ靶ＤＮＡ或ＪＥＶ感染Ｃ６／３６细胞６小时后的病毒。probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3'end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-￣(32)p]using the Klenow fragment.Thespecific radioactivity of the probe is 8.10-8.86x107cpm/μgDNA.Hybridization with this probeshowed that lpg of target DNA,or the viruses harvested from the C6/36 cell culture at 6-12 hoursafter infection with JEV,Could be detected.

关 键 词：日本脑炎病毒 寡核苷酸探针 分子杂交 合成 

分 类 号：R392－33[医药卫生—免疫学]