兔出血症病毒结构多肽分析  

The Analysis of the Structural Polyeptides of Rabbit Hemorrhagic Disease Virus

作　　者：张全顺[1] 杜念兴[1] 徐为燕[1] 

机构地区：[1]南京农业大学动物医学院,上海动植物检疫局

出　　处：《中国病毒学》1995年第3期258-260,共3页Virologica Sinica

基　　金：农业部八五课题

摘　　要：粗提病毒经Ｓｅｐｈａｒｏｓｅ４Ｂ层析后，获得纯化的兔出血症病毒（ＲＨＤＶ）。提纯病毒经过ＳＤＳ－ＰＡＧＥ经考马斯亮蓝染色显示Ａ、Ｂ、Ｃ、Ｄ、Ｅ、Ｆ和Ｇ７条多肽，凝胶扫描显示Ａ为ＲＨＤＶ主要结构多肽。用多抗和单抗作免疫转印分析，证实Ａ、Ｂ、Ｃ、Ｄ、Ｅ、和Ｇ为结构多肽，此６条结构多肽间的抗原关系十分密切。he RHDV virion comprised six sturctural polypeptides as analysed by sodium dodecylsulfate-polyacrylamide gel electrophoresis, including A(molecular weight 60.12 kD),B(58. 27kD),C(54.74kD),D(53. 01kD),E(51. 10kD) and G(25. 90kD).The major polypep-tide A(including polypeptide B constituted about 54.7% of the total viral proteins. polypep-tide C constituted 1 7.7%, polypeptide D and E were about 22.7%and polypeptide G wereabout 4.9%. By western blotting with rabbit antiserum,polypeptides A、B、C、D、E and Gwere detected. by using monoclonal antibodies AA8-1、CG4-1 and CH3-1,all the six polypep-tides could be recognized,indicating that these polypeptides shared extensive antigenic rela-tionship.

关 键 词：兔出血症病毒 结构多肽 分析 

分 类 号：S852.65[农业科学—基础兽医学] S858.291[农业科学—兽医学]