河南马氏钳蝎蝎毒素对Eca109细胞的杀伤及生长抑制作用  

The lethal and growth-inhibiting effects of the toxins existed in the scorpion venom (buthus martensii karshi)on Eca109 cells

作　　者：董伟华[1] 藏梦维 孔天翰[1] 郑智敏[1] 钱玉珍[1] 刘桂亭[1] 

机构地区：[1]河南医科大学病理生理学教研室,皖南医学院病理生理学教研室,河南医科大学人体解剖学教研室

出　　处：《中国病理生理杂志》1995年第3期251-255,共5页Chinese Journal of Pathophysiology

摘　　要：本文研究了河南马氏钳蝎蝎毒（ＳＶＣ）的三个分离毒素：ＳＶＣⅠ、ＳＶＣⅡ及ＳＶＣⅢ对Ｅｃａ１０９细胞株的杀伤及生长抑制作用。结果表明，ＳＶＣⅠ和ＳＶＣⅡ对Ｅｃａ１０９细胞有直接杀伤作用，它们对Ｅｃａ１０９细胞的半数杀伤浓度（ＬＣ＿（５０））分别为７．９９９μｇ／ｍｌ、４．４９９μｇ／ｍｌ；ＳＶＣⅡ和ＳＶＣⅢ处理细胞９６ｈ后，对Ｅｃａ１０９细胞的生长抑制率（ＩＲ％）分别达８３．４７％和９２．２９％；ＳＶＣⅡ、ＳＶＣⅢ对Ｅｃａ１０９细胞的集落抑制率分别达８１．３％、８０．１％，它们对Ｅｃａ１０９细胞的半数抑制浓度（ＩＣ＿（５０））分别为９．９００μｇ／ｍｌ、３．０９９μｇ／ｍｌ；经ＳＶＣⅡ、Ⅲ作用后，Ｅｃａ１０９细胞有丝分裂指数（ＭＩ％）亦明显减少；ＭＴＴ法的实验结果显示，ＳＶＣⅡ、Ⅲ对Ｅｃａ１０９细胞具有明显的细胞毒性作用。未观察到ＳＶＣⅠ对Ｅｃａ１０９细胞的作用。The lethal and the growth-inhibiting effect of three components (SVC Ⅰ,SVCⅡ,SVCⅢ)isolated from scorpion venom on Eca109 cells were studied. The resultsshowed that SVC Ⅱ and SVC Ⅲ possess killing effect on Eca109 cells,the half lethalconcentration(LC_(50) )of the SVC Ⅱ and SVC Ⅲ on Eca109 cells were 7.999μg/ml and 4.499μg/ml respectively. Treated the cell with SVC Ⅱ or SVC Ⅲ for 96 hours,the growth inhi-biting-rate on Eca109 cells were 83.47% and 92.29% respectively. The colony-forminginhibiting rate of SVC Ⅱ and SVC Ⅲ on Eca109 cells were 81.3% and 80.1%,and the halfinhibiting-concentration(IC_(50))were 9.900μg/ml and 3.099μg/ml respectively. The mitoticindex(MI‰)of the Eca109 cells were decreased obviously after the cells were treatedby SVCⅡ and SVC Ⅲ. The cytotoxic effect of SVC Ⅱ and SVC Ⅲ on Eca109 cells was ob-served by means of MTT test.The effect of SVCⅠ on Eca109 cells was not found.

关 键 词：食管肿瘤 细胞 马氏钳蝎 蝎毒素 药理学 

分 类 号：R282.740.5[医药卫生—中药学]