白藜芦醇甙对内毒素刺激小鼠腹腔巨噬细胞产生TNF_α和NAG的影响  

The effect of polydatin on endotoxin-activated peritoneal macrophages in mice

作　　者：张曼[1] 乐毅[1] 金丽娟[1] 

机构地区：[1]海军总医院中心实验科,第一军医大学

出　　处：《中国病理生理杂志》1995年第3期242-245,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：收集小鼠腹腔巨噬细胞，调整细胞浓度为５×１０￣５／ｍｌ，加入２４孔培养板每孔１ｍｌ，内毒素刺激前后不同时间加入白藜芦醇甙培养，检测上清中肿瘤坏死因子，溶酶体酶（Ｎ－乙酰－β－Ｄ氨基葡萄糖苷酶ＮＡＧ）的变化，结果说明：白藜芦醇甙可减少上清中ＴＮＦ＿α和ＮＡＧ的量，通过此可减少内毒素刺激产生的由ＴＮＦ＿α和ＮＡＧ介导的组织损伤。Peritoneal macrophages of mice were isolated and cultured in vitro,peri-toneal macrophages fluid(5×10￣5/ml)were incubated in 24-well tissue culture plates.1mlof the fluid were added into every well.50μl,100μl,200μl of polydatin(0.5%)wereadded respectively into these wells before and after endotoxin-stimulation. Concentrationsof TNF and NAG in the supernate were estimated.Result showed polydatin decreasedthe level of TNF and NAG in the supernate of endotoxin-stimulated macrophage.

关 键 词：内毒素 巨噬细胞 肿瘤坏死因子 白藜芦醇甙 虎杖 

分 类 号：R282.710.5[医药卫生—中药学] R363.13[医药卫生—中医学]