用体外培养模型研究硒氟对VYS结构及功能的影响  

Effect of selenium and Fluoride on structure and function of visceral yold-sac by using culture in vitro

作　　者：李勇[1] 孙棉龄[1] 吴德生[1] 王远萍[1] 杨果[1] 

机构地区：[1]成都华西医科大学环境卫生教研室,华西医科大学电镜室

出　　处：《中国地方病学杂志》1995年第3期162-165,共4页Chinese Jouranl of Endemiology

摘　　要：用体外全胚胎培养模型系统探讨了硒、氟对大鼠脏层卵黄囊（ＶＹＳ）功能和结构的影响。结果表明：随着硒、氟浓度的增加，对ＶＹＳ的损害亦逐渐增强．高浓度响（≥２．０μｇ／ｍｌ）和氟（≥１０．０μｇ／ｍｌ）可导致ＶＹＳ直径和血管形成得分值降低，ＶＹＳ三层结构变薄，微绒毛数量减少、结构不规，溶酶体数量减少，线粒体、内质网数量减少和肿胀，畸胎率增高等后果．但在一定浓度范围内（Ｓｅ０．５μｇ／ｍｌ～２．０μｇ／ｍｌ，Ｆ２．５μｇ／ｍｌ～１０．０μｇ／ｍｌ），二者呈相互桔抗作用．ＶＹＳ结构基本恢复正常，畸胎率也明显降低。结果揭示硒、氟对ＶＹＳ营养功能的损害可能是其致畸机制之一。The results showed the higher selenium and fluoride concentration, the bigger visceral yolk-sac (VYS) was damaged. At high concentration (Se≥2.0μg/ml, F≥10.0μg/ml), diameter and vasoformation score were decreased, three layers of strucatare got thinned, number of micovilli,lysosomes, mitochondria and endoplasmic reticula were also decreased, not regula microvilli and swelling mitochondria and endoplasmic reticula were found, and incidence of abnormal embryo was increased. But at the condition of 0.5μg/ml￣2.0μg/ml Se+10.0μg/ml F, selenium and fluoride had antagonism, structure of VYS were not significant difference from that of control VYS, and incidence of abnormal embryo was also decreased. These results indicate damage of selenium and fluoride on VYS nutritional function may be one of their teratogenic mechanisms.

关 键 词：脏层 卵黄囊 硒 氟 联合作用 毒理学 

分 类 号：R992[医药卫生—毒理学]