软骨型蛋白多糖的提纯及生物化学特点的研究  

Studies on biochemical characterizition and purification of cartilnge proteoglycan

作　　者：颜炜群[1] 宇莉[1] 侯立中[1] 杨同书[1] 加藤章夫 中村茂夫 牧平清超 申鸣[3] 宋国培[3] 

机构地区：[1]白求恩医科大学应用基础医学研究所 [2]日本广岛大学齿学部生物化学教研室 [3]白求恩医科大学第一临床医院内科

出　　处：《中国地方病学杂志》1995年第3期153-156,共4页Chinese Jouranl of Endemiology

摘　　要：本文分别用Ｑ－Ｓｅｐｈａｒｏｓｅ柱层析和氯化铯平衡密度梯度离心法从仔猪软骨提纯了软骨型蛋白多糖单体，它在ＳｅｐｈａｒｏｓｅＣＬ－２Ｂ柱层析上显示了大分子的特点（Ｋａｖ＝０．２２）．能够与透明质酸结合形成巨大分子的聚合体。提纯的核心蛋白在ＳＤＳ电泳上显示出单一的银染色区带，分子量为４６０ｋＤａ，与抗人蛋白多糖单克隆抗体（３－Ｂ－３）呈强阳性反应．离子交换层析法简便、快速、经济更适合于以蛋白多糖损害为主要特点的软骨疾病的研究。The large cartilage-specific proteoglycans were purified from pig chondrocyte cultured using method of Q-Sepharose ion exchange chromatography or isopycnic CsCl density gradient centrifugation. The purified proteoglycanm showed the large (90K, Kav=0.22) and small (10%) of hydrodynamic size on Sepharose CL-2B chromatography. Core polypeptide from large proteoglycan (generated by digestion with chondroitinase and keratanase) was purified on Superose 6B chromatography. It showed silverstained single band of 460kDa on SDS polyacylamide gel electrophoresis, and strongly reactivity with an antiserum to human proteoglycan in Western blood. This fast, simple, and low cost method of Q-Sepharose chromatography produces high quality proteoglycans, suitable for studies on proteoglycan in the injured cartilage.

关 键 词：蛋白多糖 核心蛋白 软骨疾病 提纯 生物化学 

分 类 号：Q539[生物学—生物化学] R681.3[医药卫生—骨科学]