人红细胞对溴甲烷代谢的实验研究  

An Experimental Study on the Metabo-lism of Methyl Bromide by HumanErythrocyte

作　　者：张亚飞[1] 徐代根[1] 陈萍[1] 贺涵贞[1] 

机构地区：[1]深圳市沙头角卫生防疫站,同济医科大学职业医学研究所

出　　处：《中国工业医学杂志》1995年第2期77-79,共3页Chinese Journal of Industrial Medicine

基　　金：国家自然科学基金育年基金

摘　　要：研究了人红细胞对溴甲烷的代谢发现，约９２％的人红细胞对溴甲烷具有酶促代谢作用，比国外报道的６０％要高，并且Ｐｂ￣（２＋），Ｃｄ￣（２＋）和Ｈｇ￣（２＋）均能抑制该酶促代谢作用，有明显的剂量－反应关系。未发现三种离子抑制作用之间的差别。可用此方法对职业性接触一卤代甲烷的人群进行筛选，以保护工人健康。对暴露Ｐｂ￣（２＋），Ｃｄ￣（２＋）和Ｈｇ￣（２＋）的职业人群可通过检测谷胱甘肽Ｓ－甲基转移酶Ｒｈｏ（ＧＳＨ－Ｓ－ＴｒＲｈｏ）来反映机体接触水平及其对机体的损伤程度。Metabolism of methyl bromide by humanerythrocytes plasma was studied and thedecline in concentration of methyl bromide was monitored by gas chromatography-every15 minutes,About 92% of the subjects show-ed metabolism of methyl bromide in theirerythrocytes, suggesting that the majorityof the Chinese population have glutathione S-transferase Rho,the rate is much higherthan that in European population as reportedby some authors .Pb￣(2+),Cd￣(2+) and Hg￣(2+) couldinhibit the enzymaticc metatolism and the inhibition was dose dependent, althoughno significant difference in their inhibitiveeffect was found.The measurement ofglutathione S-transferase Rho activity canbe applied to biom on itor the workers occupa-tionally exposed to monohalogenated meth-anes.The inhibition of the enzyme by Pb￣(2+),Cd￣(2+)and Hg￣(2+) may represent the level of exposure to theSe ions and the damage to thebody.

关 键 词：职业危害 溴甲烷 红细胞 

分 类 号：R598.1[医药卫生—内科学]