不同龄SHR心肌细胞内Ca^（2＋）及心肌组织NE含量的变化  

Changes in Myocardial Intracellular Calcium Concentration and Myocardium Norepinephrine Content in Spontanously Hypertensive Rats of Various Ages

作　　者：王军[1] 章鲁[1] 齐建华[1] 张佩瑾[1] 顾培堃 魏丕敬[1] 金正均[1] 

机构地区：[1]上海第二医科大学药理学教研室,上海第二医科大学医用计算机教研室,上海第二医科大学电生理技术教研室

出　　处：《中国高血压杂志》1995年第2期104-106,共3页

基　　金：国家自然科学基金

摘　　要：采用荧光探针结合计算机图像处理技术测定幼年、成年、老年ＳＨＲ心肌单细胞内游离Ｃａ^（２＋）浓度，采用高压液相色谱法测定幼年、成年、老年ＳＨＫ心肌去甲肾上腺素（ＮＥ）含量。结果显示随着年龄增加，ＳＨＲ心肌细胞内游离Ｃａ^（２＋）增加，而心肌组织ＮＥ含量下降。与老年ＷＫＹ大鼠比较，老年ＳＨＲ心肌细胞内Ｃａ（２＋）含量较高（Ｐ＜０．０１），而其心肌组织ＮＥ含量却较少（Ｐ＜０．０５）。本研究提示心肌组织ＮＥ与ＳＨＲ的血压升高及心肌细胞内游离Ｃａ^（２＋）增多无直接关系。＊Ｐ＜０．０１，ａ．ｖｓ．ＳＨＲ２ｍ，ｂ．ｖｓＳＨＲ６ｍ，ｃ．ｖｓ．ＳＨＲ１２ｍ３不同年龄阶段ＳＨＲ心肌组织ＮＥ含量ＳＨＲ的年龄越大，其心肌组织ＮＥ的含量越少，各年龄组之间均有显著差异（Ｐ＜０．０１）。与老年ＷＫＹ大鼠比较．老年ＳＨＲ是心肌组织ＮＥ含量明显降低（Ｐ＜０．０５）（Ｆｉｇ３）。Ｆｉｇ３ＮＥｃｏｎｔｅｎｔｓｉｎｃａｒｄｉａｃｔｉｓｓｕｅｓＩｎＳＨＲｓｏｆｖａｒｉｏｕｓａｇｅｓａｎｄｅｌｄｅｒＷＫＹｒａｔｓ：Ｐ＜０．０５．＊：Ｐ＜０．０１，ａ．ｖｓ．ＳＨＲ２ｍ，ｂ．ｖｓＳＨＲ６ｍ．ｃ．ｖｓＳＨＲ１２ｍ．Fluorescent probe combined with computer image processing technique was used to detect intracellular calcium in myocytes in young, adult and aged SHR,HPLC was used to measure NE content in cardiac tissues SHR of various ages.The results showed that the myocardial intracellular free calcium concentrations increased but NE content decreased in SHR with age. The intracellular calcium concentration in aged SHR myocytes was higher than that in aged WKY rats(P<0.05).On the contrary,NE contents were lower(P<0.05).It is suggested that there is no direct relation between NE contents in cardiac tissues and elevated blood pressure of genetic SHR as well as intracellular free Ca(2+)in myocytes.

关 键 词：高血压 心肌细胞 钙 去甲肾上腺素 

分 类 号：R544.102[医药卫生—心血管疾病]