McAb4F8、McAb1F11与囊尾蚴抗原作用免疫印渍分析  

RECOGNITION OF CYSTICERCUS ANTIGENS BYIMMUNOBLOTTING WITH McAb4F8 and McAb1Fll

作　　者：张洪花[1] 王昌源[1] 刘湘萍[1] 王世君 王辉东[1] 刘玉冰[1] 刘伦华[1] 

机构地区：[1]山东省寄生虫病防治研究所,山东省莱西县孙受医院

出　　处：《中国寄生虫病防治杂志》1995年第2期105-107,共3页Chinese Journal of Parasitic Disease Control

摘　　要：将猪囊尾蚴囊液抗原（ＣＣＦＡ）和脑囊虫病病人脑脊液（ＰＣＳＦ）进行ＳＤＳ－ＰＡＧＥ电泳分析，发现在ＣＣＦＡ中有１４条蛋白带，范围在２８～７６ｋＤａ。脑囊虫病病人ＣＳＦ中有６条蛋白带，范围在３３～８３ｋＤａ。用ＣＣＦＡ和ＰＣＳＦ与ＭｃＡｂ４Ｆ８和ＭｃＡｂ１ＦＩ１１免疫印迹试验，证明ＣＣＦＡ中５０、５３、６３和７０ｋＤａ蛋白带与两株ＭｃＡｂ均有反应，另一条蛋白带３３ｋＤａ仅能被ＭｃＡｂ１Ｆ１１识别。ＰＣＳＦ中的６条蛋白带中有３条（５３，６３和７０ｋＤａ）与２株ＭｃＡｂ均有反应，另一条蛋白带３３ｋＤａ仅与ＭｃＡｂ１Ｆ１１反应。２例对照脑脊液中未出现反应带。结果显示ＭｃＡｂ４Ｆ８和ＭｃＡｂ１Ｆ１１均抗ＣＣＦＡ和ＰＣＳＦ中的主要蛋白带。The protein profile of cysticercus cystfluid antigens (CCFA) and that of cere-brospinal fluid(CSF) from patients with neu-rocysticercosis (PCSF) were analysed by SDS-PAGE. Fourteen hands between 28 kDa and76kDa in CCFA and 6 hands ranging from 33kDa to 83 kDa in PCSF were found- Im-munoblotting using CCFA and PCSF forMcAb4F8 and McAblFll demonstrated thathands 50 , 53 , 63 and 70 kDa in CCFA reactedwith both McAb4F8 and McAb1Fll. Anotherband 33 kDa was only recognised hyMcAblFll. Of 6 hands in PCSF, 3 hands( 53, 63 and 70 kDa) reacted with bothMcAb4F8 and McAhlFll, and hand 33 kDawas only reactive to McAblFll. No reactiveband was found with CSF of 2 controls, pa-tients with cerebral haemorrhage. The resultsshowed that both McAb4F8 and McAb1Fllwere against major proteinous antigens in CC-FA and PCSF.

关 键 词：脑囊虫病 囊尾蚴囊液 抗原 脑脊液 免疫印迹 

分 类 号：R532.330.3[医药卫生—内科学]