应用斑点ELISA检测华支睾吸虫病患者循环抗原的研究  

STUDY ON THE DETECTION OF CIRCULATING ANTIGEN IN PATIENTS WITH CLONORCHIASIS SINENSIS BY DOT-ELISA

作　　者：张月清[1] 温艳[1] 周茉[1] 吴赵永[1] 甘绍伯[1] 

机构地区：[1]北京热带医学研究所,世界卫生组织并殖吸虫病和华支睾吸虫病合作中心

出　　处：《中国寄生虫病防治杂志》1995年第1期51-53,共3页Chinese Journal of Parasitic Disease Control

摘　　要：用斑点ＥＬＩＳＡ检测１４０例粪检虫卵阳性的华支睾吸虫病人血清中的循环抗原（ＣＡｇ），阳性率为９７．１％。在１００例正常人和１０２例其它７种寄生虫病人中，有１．０％假阳性和２．０％轻度交叉反应。对６６例华支睾吸虫病人经吡喹酮治疗后１～２月进行ＣＡｇ复查，有２９例（４３．９％）转为阴性，即使仍保持阳性反应者，其反应强度亦有明显降低。用本法进行流行病学调查，获得较满意的结果，检测血清中ＣＡｇ与粪便中虫卵阳性和阴性总符合率高达９５．５％。Sera of 140 patients with clonorchiasis sinensis diagnosed by parasitological exam-ination were examined for circulating antigen with Fast-Dot-ELISA. The positive rate wasfound to be 91 1(1.0%)of 100 normal healthy subject's sera gave false positive reactio.2(2.0%)of 102 cases of various other parasitic diseases , including schistosomiasis , paragonimia-sis , hookworm infections,cysticercosis ,filariasis ,giardiasis,amoebiasis showed weak across re-action. 66 patients were treated with praziquantel and their CAg were examined 1-2months af-ter the treatment.29(43.9%)of these sera turned to be negative,the rest sera,though still re-mained positive, became markedly reduced. The survey was made in Jilin Province Jiutai county.The total consistency rate between CAg in sera and presence of eggs in fecal specimens was 95.5%。 These results indicated that circulating antigen detection is useful in the diagnosis of activeclonorchiasis as well as in the evaluation of its drug therapy.

关 键 词：华支睾吸虫病 循环抗原 酶联免疫测定法 

分 类 号：R532.230.3[医药卫生—内科学]