化学合成恶性疟原虫杂合抗原基因表达产物免疫功能的研究  

STUDY ON THE IMMUNE FUNCTION OF THEEXPRESSED PRODUCTS OF CHEMICALLY一SYNTHESIZEDHYBRID ANTIGEN GENE OF PLASMODIUM FALCIPARUM

作　　者：陈仕荣[1] 胡亚芳[1] 钟雄林[1] 王昌才[1] 马维芳[1] 郭茂云 王启松[1] 谢毅[1] 

机构地区：[1]第一军医大学分子生物学研究所,复旦大学遗传学研究所

出　　处：《中国寄生虫病防治杂志》1995年第4期247-251,共5页Chinese Journal of Parasitic Disease Control

摘　　要：本文报道化学合成恶性疟原虫杂合抗原基因３个重组克隆菌表达产物，经ＰＡＧＥ纯化后，免疫家兔制备血清，进行琼脂双向扩散和酶联免疫吸附试验及Ｗｅｓｔｅｒｎｂｌｏｔ和疟原虫体外生长抑制试验，结果表明化学合成恶性疟原虫杂合抗原基因表达产物均具有良好的免疫原性及明显的抑制其原虫体外生长的作用。The chemically一synthesized hybrid gene of P. falciparum was recombined with plas-mid pWR450.1 and expressed to produceβ galactosidase一hybrid polypeptide fusion protein in E.coli.The products were usedto immunize rabbits to prepare antisera which were analysed by immun-odiffusion in agar and ELISA and used for the inhibition of the growth of in vitro 一cultured P. falci-parum.Results showed that the gene could express fusion protein with immunogenicity and the antis-era obtained could effectively inhibit the growth of P. falciparum in vitro.

关 键 词：疟原虫 恶性疟原虫 基因表达 免疫血清 化学合成 

分 类 号：R382.31[医药卫生—医学寄生虫学]