体外测定恶性疟原虫对七种抗疟药的敏感性  

SENSITIVITY OF PLASMODIUM FALCIPARUM TO SEVEN ANTIMALARIALS IN CHINA LAOS BORDER 

作　　者：杨恒林[1,2] 刘德全[1,2] 董莹[1,2] 杨品芳[1,2] 刘瑞君[1,2] 詹斌[1,2] 张春勇[1,2] 

机构地区：[1]云南省疟疾防治研究所 [2]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1995年第2期111-113,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：联合国发展规划署/世界银行/世界卫生组织热带病研究培训特别计划处;中国预防医学科学院科学基金;卫生部青年科学基金

摘　　要：１９９２年应用体外微量法在中老边境测得我国境内恶性疟原虫对氯喹、哌喹、青蒿琥酯、蒿甲醚、蒿乙醚、还原青蒿素及咯萘啶抗性率，分别为９７．０％、９６．４％、１２．１％、１６．０％、６．２％、１２．５％、３４．５％；半数抑制量（ＩＤ５０）依次为１１９．０、３２０、７．２、２９５．０、７４．４、５．４及３１．９ｎｍｏｌ／Ｌ。老挝境内恶性疟原虫分离株对氯喹、哌喹及咯萘啶的抗性率分别为９／１０、８／１０及１／５；ＩＤ５０依次为１１４．０、１６６．９及１６．４ｎｍｏｌ／Ｌ；对青蒿琥酯、蒿甲醚、蒿乙醚及还原青蒿素均敏感，ＩＤ５０分别为５．０、９１．６、５６．７及４．４ｎｍｏｌ／Ｌ。结果提示境内外恶性疟原虫株对氯喹的抗性程度相似，但对哌喹的抗性程度境外明显低于境内。对青蒿琥酯、蒿甲醚、蒿乙醚及还原青蒿素的敏感性境外明显高于境内。The sensitivity of P.falciparum to chloroquine(CHL),piperaquine(PIP),artesunate(ATS),dihydroartemisinine(DAT),artemether(ATM),arteether(ATE) and pyronaridine(PRN)were assayed by in vitro microtechnique in China Laos border in 1992. The resistance rates of isolates from the south of Yunnan of China in the border were 97.0%,96.4%, 12.5%, 16.0%,6.3% and 34.5%,respectively;ID 50 were 119.0,320 0,7.2,5.4,295.0,74.4 and 31.9 nmol/L,ID 95 were 625.0,2426.0,42.2,36.6,767.0,371.1 and 325.2 nmol/L.The resistance rates of isolates from the north border of Laos were in order 9/10,8/10,1/5 to CHL,PIP,PRN,ID 50 were 114.0, 166.9,16.4 nmol/L,ID 95 were 570.0, 631.1 and 107.7 nmol/L. All the isolates from Laos were sensitive to ATS,DAT,ATM and ATE.ID 50 were 5.0,4.4,91.6 and 56.7 nmol/L,ID 95 were 23.4,11.1, 3276.1 and 223.9 nmol/L,respectively. The results indicate that P.falciparum in the border was highly resistant to CHL and PIP, while most of the isolates were susceptible to the other 5 of the above mentioned drugs. A comparison between the isolates from China and Laos showed that there was no significant difference in resistance degree to CHL but the sensitivity of isolates from China to other 6 drugs was lower than those from Laos.

关 键 词：疟原虫 恶性 测定 氯喹 哌喹 青蒿素类 咯萘啶 

分 类 号：R531.305[医药卫生—内科学] R978.61[医药卫生—临床医学]