滤纸干血滴抽提恶性疟原虫 DNA 用于 PCR 扩增  

APPLICATION OF PLASMODIUM FALCIPARUM DNA EXTRACT FROM DRIED BLOOD SPOT OF FALCIPARUM MALARIA PATIENT IN POLYMERASE CHAIN REACTION

作　　者：张龙兴[1] 詹斌[1] 王聚君[1] 冯晓平[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1995年第2期120-122,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：以ＳＴＥ－蛋白酶Ｋ－ＳＤＳ、甲醇－蛋白酶Ｋ－ＳＤＳ、Ｃｈｅｌｅｘ－１００加热法和Ｃｈｅｌｅｘ－１００蛋白酶Ｋ等四种方法对恶性疟患者滤纸干血滴样品进行ＤＮＡ抽提，供ＰＣＲ扩增特异性ＡＭＡ－１ＤＮＡ片段。结果显示，除ＳＴＥ－蛋白酶Ｋ－ＳＤＳ法抽提ＤＮＡ进行ＰＣＲ试验未能获得扩增产物外，其他３种方法抽提ＤＮＡ后进行ＰＣＲ试验均获得特异性约９００ｂｐＤＮＡ片段，可供进一步试验。Polymerase chain reaction (PCR) primered with primers 3,4 and 5,6 was performed using DNA extracted from dried blood spot of a falciparum malaria patient from Mengpeng Township,Yunnan using four different methods,including STE proteinase K SDS,methanol proteinase K SDS,Chelex 100 and Chelex 100 proteinase K to amplify DNA of apical membrane antigen 1(AMA 1).A 900 bp DNA band could be seen on the ethidium bromide stained agarose gel electrophoresis of the PCR products when DNA was extracted using all the above mentioned methods except STE proteinase K SDS method. DNA extracted with Chelax 100 was recommended.

关 键 词：滤低干血滴 恶性 疟原虫 基因 聚合酶链反应 

分 类 号：R382.31[医药卫生—医学寄生虫学]