多重耐药大肠杆菌β－内酰胺酶的初步研究  

Preliminary studies on β-lactamases of a multi-resistant E,coli strain

作　　者：刘必刚[1] 包定元[1] 范听建[1] 舒煦 

机构地区：[1]四川肿瘤研究所,华西医科大学药理教研室,华西医科大学药理及第一附属医院

出　　处：《中国抗生素杂志》1995年第5期340-344,共5页Chinese Journal of Antibiotics

摘　　要：用Ｎｉｔｒｏｃｅｆｉｎ法检测到多重耐药大肠杆菌ＨＸ８８１０８及其４株质粒转化菌内均含有β－内酰胺酶；酶稳定性实验显示大肠杆茵ＨＸ８８１０８的４个质粒所编码的β－内酰胺酶对８种β－内酰胺类抗生素的水解享及对头孢哌酮的水解扫描光谱各不相同；ＰＡＧＥ实验表明大肠杆菌ＨＸ８８１０８内含有几条β－内酰胺酶条带，４个质粒编码的β－内酰胺酶条带在凝胶上的位置互不相同，但都与大肠杆菌ＨＸ８８１０８的１条β－内酰胺酶条带的位置相同。以上结果提示：大肠杆菌ＨＸ８８１０８内的４个质粒编码４种不同的β－内酰胺酶，从而介导大肠杆菌ＨＸ８８１０８对多种β－内酰胺类抗生素耐药。By means of nitrocefin,β-lactamase were detected in a multi-resistant E.coli strain,E. coli HX88108 and its 4 plasmid transformants. The β-lactamase stability testshowed that the hydrolysis rates of 8 tested β-lactam antibiotics with the β-lactamasesencoded by the 4 plasmids were different;and their hydrolysis spectrum of cefoperazone werenot similar either.PAGE experiment revealed that E.coli HX88108 contained several β-lactamase bands,and band location of the β-lactamases encoded by the 4 plasmids weredifferent but each was same as one of β-lactamase bands of E. coli HX88108.These resultsdemonstrated that 4 plasmids of E. coli HX88108 encoded 4 different β-lactamases andmediated the strain exhibiting strongly resistance to β-lactam antibiotics.

关 键 词：大肠杆菌 Β内酰胺酶 质粒 抗药性 

分 类 号：R915[医药卫生—微生物与生化药学]