同种异体小鼠淋巴细胞杂交瘤腹水的诱生  

THE INDUCTION OF ASCITES BY MOUSE ALLOGENEIC LYM-PHOCYTE HYBRIDOMAS

作　　者：董杰 季永镛[1] 

机构地区：[1]中国科学院上海细胞生物学研究所

出　　处：《中国免疫学杂志》1995年第2期75-77,共3页Chinese Journal of Immunology

摘　　要：本文研究了分泌抗天花粉蛋白ＩｇＥ抗体的同种异体小鼠杂交瘤株（Ｂａｌｂ／ｃ×Ｃ５７ＢＬ／６Ｊ）ＴＥ１的腹水的诱生，最佳诱生腹水的宿主是遗传背景相容的Ｆ１（Ｂａｌｂ／ｃ×Ｃ５７ＢＬ／６Ｊ）小鼠，其次是与杂交瘤亲本骨髓瘤细胞ＮＳ－１遗传背景一致的Ｂａｌｂ／ｃ小鼠，同时比较了各种免疫抑制处理宿主的腹水诱生率，其中以照光处理最佳，此外对杂交瘤株进行“驯化”，得到了易于诱生腹水并且抗体效价高的杂交瘤细胞。he induction of ascites by inoculation of mouse allogeneic hybridomas (Balb/c × C57BL/6J)secreting IgE antibodies to trichosanthin was studied. The optimal hosts for ascites inductionwere mice with same genetic backgroun, F1 (Balb/c×C57BL/6J)mice, The next was Balb/cmice,with genetic background same to NS-1 cells. The ascites induction rate of several methodsfor immunosuppression of the host mice were also compared.Of them irradiation came to thefirst. Furthermore through “training”proesses,hybridoma cells liable to induce ascites and secret-ing high titer antibodies could be obtained.

关 键 词：腹水 诱生 杂交瘤 淋巴细胞 

分 类 号：R392.12[医药卫生—免疫学]