鼠抗丙型肝炎病毒（HCV）NS3区单克隆抗体的研制及特性分析  

PKEPARATION AND CHARACTERIZATION OF MURINE MONO-CLONAL ANTIBODY AGAINST NS3 REGION OF HEPATITIS CVIRUS

作　　者：孟淑芳[1] 李秀华[1] 张宁[1] 尹红章[1] 李德富[1] 

机构地区：[1]中国药品生物制品检定所

出　　处：《中国免疫学杂志》1995年第5期278-279,289,共3页Chinese Journal of Immunology

摘　　要：用淋巴细胞杂交瘤技术，制备出５株抗ＨＣＶ－ＮＳ３区单克隆抗体（ＭｃＡｂ），分别命名为Ｃ７－１，３，６，８，９。单抗特性测定结果显示５株单抗均为ＨＣＶ－ＮＳ３区特异性，与ＨＣＶ其它区域的抗原及宿主茵成份均无交叉反应；此５株单抗识别ＮＳ３区上两个不完全相关的抗原位点，其中Ｃ７－１，３，６株识别同一抗原决定簇；Ｃ７－９的识别位点与此３株有一定的相关；Ｃ７－８则识别与此４株不完全相关的位点。ＨＣＶ－ＮＳ３抗体阳性病人血清对３株单抗显示不同程度的竞争抑制作用，揭示自然抗原上存在这两个不完全相关的抗原位点，并为单抗进一步分析ＨＣＶ自然抗原及感染提供了理论基础。Five hybridomas, named C7-1,3,6,8 ,and 9 were established by hybridoma technique.Characterization of these hybridomas demonstrated that these five monoclonal antibodies(McAbs)were all specific for HCV-NS3 antigen and had no cross-reactivity with any other anti-gens of HCV and E. coli components. They recognized two incomplete-related epitopes on NS3antigen. HCV-NS3 antibody positive sera inhibited the binding of McAbs to antigen with differ-ent extent.These results indicated that there were two epitopes on natural antigen of HCV asthe same as th on engineering antigens. It also shows that McAb to HCV is useful to analysis ofHCV antigens and study of HCV infection.

关 键 词：单克隆抗体 丙型肝炎病毒 制备 

分 类 号：R392－33[医药卫生—免疫学] R512.630.5[医药卫生—基础医学]