应用RFLP图谱定位分析籼稻粒形数量性状基因座位  

RFLP MAPPING OF QTLs FOR GRAIN SHAPE TRAITS IN INDICA RICE(ORYZA SATIVA L.SUBSP.INDICA)

作　　者：林鸿宣 闵绍楷[1] 熊振民[1] 钱惠荣[1] 庄杰云[1] 陆军[1] 郑康乐[1] 黄宁 

机构地区：[1]中国水稻研究所

出　　处：《中国农业科学》1995年第4期1-7,共7页Scientia Agricultura Sinica

基　　金：国家"863"高技术计划项目;浙江省自然科学基金;洛氏基金

摘　　要：用二个籼型杂交组合的Ｆ_２群体，构建了二张分别具有８９和９３个标记位点的ＲＦＬＰ图谱。在此基础上，应用方差分析法和区间作图法对控制籼稻粒形性状的ＱＴＬｓ进行定位。在特三矮２号／ＣＢ１１２８群体中，定位了１４个ＱＴＬｓ，其中５个控制粒长，２个主效基因（分别位于第５、第７染色体）和２个微效基因控制粒宽，１个主效基因和４个微效基因控制粒厚。在外引２号／ＣＢ１１２８群体中，共定位了１３个ＱＴＬｓ，其中５个影响粒长，２个主效基因（分别位于第２、第５染色体）和３个微效基因控制粒宽，３个微效基因影响粒厚。此外，估算了每个ＱＴＬ的贡献率、加性效应和显性效应值，分析某些染色体区间的多效现象，比较分析二群体ＱＴＬｓ定位结果的异同。Based on two RFLP maps each with 89 and 93 marker loci from two F2 populations,QTLs for grain shape traits have been identified and mapped via analysis of variance and interval mapping.In Tesanai 2/CB1128 population,14 QTLs were detected,5 for the length of grain,2 major genes(one located on chromosome 5,6 cM from RG182,the other on chromosome7,5 cM from RG4)and 2 minor genes for the width of grain,1 major gene and 4 minor genes for the thicknessof grain.While in Waiyin 2/CB1128 population,13 QTLs were detected,5 for the length of grain,2 major genes(on chromosome 2 and 5 respectively)and 3 minor genes for the width of grain,3 minor genes for the thickness of grain. In addition,the % variation explained,additive effects and dominant effects for all QTLs detected were estimated,and pleiotropic effects on some regions of chromosomes have been analyzed,comparisons between the populations for all the QTLs detected were performed.

关 键 词：籼稻 RFLP 粒形性状 QTL定位 

分 类 号：S511.21[农业科学—作物学]