贝氏隐孢子虫在鸡胚绒毛尿囊膜上发育过程的研究  

THE LIFE-CYCLE OF CRYPTOSPORIDIUM BAILEYI IN THE CAM OF CHICKEN EMBRYO

作　　者：赵亚荣[1] 宋生贵[1] 张晋英[1] 张玉换[1] 蒋金书[1] 

机构地区：[1]山西省农业科学院畜牧兽医研究所,北京农业大学兽医学院

出　　处：《中国农业科学》1995年第6期83-89,共7页Scientia Agricultura Sinica

摘　　要：借助微分干涉显微镜（ＮＩＣ）对贝氏隐孢子虫（Ｃ．ｂａｉｌｅｙｉ）在鸡胚ＣＡＭ上的发育过程进行了研究。整个发育周期约为９６小时，全部发育过程包括：脱囊－裂殖生殖－配子生殖－孢子生殖，共４个阶段。裂殖生殖共产生３代裂殖体，在第Ⅰ、Ⅲ代裂殖体中分别含有８个裂殖子，在第Ⅱ代裂殖体中含有４个裂殖子。孢子化过程在ＣＡＭ内胚层上皮细胞表面的带虫空泡内完成，并产生薄壁型和厚壁型两种卵囊。首次提出虫体具有群体寄生现象和同步发育特征；描述了Ｃ．ｂａｉｌｅｙｉ的运动期虫体离开母体和发育为滋养体的动态变化过程；应用由粪便中纯化的卵囊直接感染鸡胚成功。并用３７℃水浴脱囊，脱囊率达６０％左右，表明温度对隐孢子虫的脱囊具有重要作用，无需胰酶和牛胆酸钠的参与。动物回归试验表明：Ｃ．ｂａｉｌｅｙｉ不能经胚胎途径造成感染，在ＣＡＭ上发育的Ｃｂａｉｌｅｙｉ卵囊对雏鸡具有较强的感染性。The life-cycle of C.baileyi in the CAM of chicken embryo was studied by the Nomarski interference-contrast microscopy(NIC).It included excystation, merogony(with three generations),gamogony and sporogony.The first and the third generation meronts contained 8 merozoites separately,and the second generation contained 4 merozoites.The sporogony process occurred in the parasitophous vaccuole (PV)which was located in the epithelial cell of CAM, and resulted in two types of oocysts:thin-walled oocyst and thick -walled oocyst .The authors first reported the social phenomenon and synchronizing development in C. baileyi,the dynamic Process of the sporozoites or merozoites apart from their parent substances and developed into trophozonts,the culture of C.baileyi in chicken embryo with oocysts directly,and the excysting process without both the trypsin and the sodium taurocholate in 37℃ water bath.The high level of excystation (60%) happened at 37 ℃ water,which showed that temprature was very important in C. baileyi excysting process.Ooryst obtained from chicken embryo caused heavy infections of chicken in BF, cloaca and respiratory tract.It also showed that the infection in chicken did not take place through the embryo directly.

关 键 词：贝氏隐孢子中 鸡胚培养 动物回归试验 

分 类 号：S852.723[农业科学—基础兽医学]