空肠弯曲菌蛋白抗原分析  

Analysis of Protein Antigens of Campylobacter jejuni

作　　者：臧星星[1] 马宝骊[1] 曹鹤年 黄冬生[1] 柏峻[1] 王利[1] 谢雅莉[1] 

机构地区：[1]上海第二医科大学,上海市免疫学研究所

出　　处：《中国人兽共患病杂志》1995年第2期2-6,共5页Chinese Journal of Zoonoses

摘　　要：超声粉碎和高速离心获得Ｃ．ｊ－Ｓ１３１ｌｙｓａｔｅ，ＳＤＳ－ＰＡＧＥ显示位于１０－９２ＫＤ间２４条蛋白条带，薄层扫描发现４３ＫＤ蛋白是菌体最主要成份，其次是１５ＫＤ和１７ＫＤ蛋白。Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ发现Ｃ。ｊ－Ｓ１３１ｌｙｓａｔｅ中４３ＫＤ蛋白抗原性最强，也最早诱导相应抗体产生。用凝胶过滤和液相制备型等电聚焦分离获得了纯化的９２ＫＤ、４３ＫＤ、１５ＫＤ三种蛋白。本文结果为进一步研究空弯菌诱致自身免疫综合征的分子机理打下了基础。The 1ysate derived from Campylobacter jejuni, strain C.j-S131, contained at least 24 protein bands with molecular weight from 10KD to 92KD, examined by SDS-PAGE. Chromatogram scanning showed that 43KD,15KD and 17KD proteins were major components. Western blotting indicated that 43KD protein was dominant antigen and that specific antibody against 43KD protein appeared earliest. Three proteins, 92KD, 43KD, 15KD, were purified by gel chromatograph and preparative scale-free solution-isoelectric focussing, our results provided basis for further research of molecular mechanisms of C.j-S131-induced autoimmune syndrome.

关 键 词：空肠弯曲菌 蛋白抗原 

分 类 号：R378.2[医药卫生—病原生物学]