非同位素标记DNA探针与恶性疟原虫核酸杂交的实验研究  

Studies on Nonisotope-labeling DNA Probes and Its Hybridization with Plasmodium falciparum Nucleic Acids

作　　者：余新炳[1] 方建民[1] 

机构地区：[1]中山医科大学寄生虫学教研室,广州510089

出　　处：《中国人兽共患病杂志》1995年第6期13-15,共3页Chinese Journal of Zoonoses

摘　　要：应用聚合酶链反应扩增恶性疟原虫环子孢子蛋白质(CSP)基因,扩增产物使用随机引物法进行地高辛标记,所制备的CSP基因探针斑点杂交试验表明,至少可检测出25个单纯虫体,可探及的最低DNA限量为5pg水平。Southern印迹杂交结果分析表明,CSP基因探针可检测出虫体最低DNA水平为6.4pg。In this report, the gene of circumsporozoite protein (CSP) of Plasmodium falciparum were amplified by polymerase chain reaction (PCR) . And PCR products were labeled with digoxigenin by the random primer technique. The result of dot-blotting hybridization assay showed that CSP gene probes could detect at least 25 malaria parasites and 5 pg DNA from Plasmodium falciparum. Also, Southern-blotting hybridization assay indicated that CSP gene probes could detect 6- 4pg DNA. It was evident that CSP gene probes were possessed of a high degree of sensitivity.

关 键 词：疟原虫 环子孢子 蛋白质 探针 DNA 核酸杂交 

分 类 号：R382.31[医药卫生—医学寄生虫学]