用单克隆抗体结合酶联免疫吸附试验检测棉铃虫幼虫体内的核多角体病毒  

USE OF MONOCLONAL ANTIBODIES AND ELISA TO DETECT THE NUCLEAR POLYHEDROSISVIRUS (NPV) IN THE LARVAE OF HELICOVERPA ARMIGERA

作　　者：陆自强[1] 胡广淦[1] 陈丽芳[1] 杨峰[1] 杭三保[1] 乐云仙[2] 苏德明[2] 

机构地区：[1]扬州大学农学院植保系 [2]复旦大学生命科学学院

出　　处：《中国生物防治》1995年第1期36-39,共4页Chinese Journal of Biological Control

基　　金：国家自然科学基金

摘　　要：在常规ＥＬＩＳＡ间接法的基础上，应用单克隆抗体检测感染棉铃虫核型多角体病毒的棉铃虫幼虫体内病毒粒子。３龄幼虫饲喂表层涂有ＨａＮＰＶ人工饲料后９小时，即可在幼虫抽提液中检出病毒粒子抗原，而典型病虫显症需５～６天后才出现。因此本法是一种灵敏、快速、特异性强的检测昆虫杆状病毒的方法。利用单克隆抗体结合ＥＬＩＳＡ试验分别检测来自江苏和山东不同地区棉田自然死亡的棉铃虫幼虫，表明在江苏和山东不同棉区均可检测到ＨａＮＰＶ病毒粒子。An indirect enzyme linked immunosorbent assay( ELISA) incorporating monoclonal antibody was used to detect virus antigen in NPV infected H. armigera larvae. Third instars were fed with artificial diet contaminated on the surface with the NPV virus antigen was detected in larval extract at 9 hou r after exposure to the virus whereas disease symptom was observed after 5～6 days. ELISA has been shown to be a rapid, specific and senstive method for dete cting and quantifying baculovirus. The corpse of H. armigera from the field of both Jiangsu and Shandong province, was detected by ELISA. The result showe d that HaNPV could be detected in various regions in Jiangsu and Shandong.

关 键 词：单克隆抗体 棉铃虫 核型多角体病毒 ELISA 检测 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治]