HCG酶免疫电极的现场FTIR反射光谱  

IN SITU FTIR REFLECTION SPECTR0SCOPYOF HCG ENZYME IMMUNOELECTRODE

作　　者：董飒英[1] 吕鸣祥[1] 黄振才[1] 

机构地区：[1]天津大学应用化学系,军事医学科学院微生物流行病研究所

出　　处：《中国生物医学工程学报》1995年第2期107-112,共6页Chinese Journal of Biomedical Engineering

基　　金：厦门大学固体表面物理化学国家重点实验室

摘　　要：利用现场ＦＴＩＲ反射光谱研究了电子中继物（介体）型ＨＣＧ酶免疫电极在不同电极电位下的电位差谱及同一电位不同状态下的单光束光谱之差。讨论了ＨＣＧ（人绒毛膜促性腺素）、抗ＨＣＧ（ａｎｔｉ－ＨＣＧ）及它们的络合物在电极表面的耦联情况和红外光谱特性。对两种不同方法制备的ＨＣＧ免疫电极的谱图加以对比和解析，并初步确定了ａｎｔｉ－ＨＣＧ的特征谱峰。A new method of in-situ FTIR reflection spectroscopy was used instudying potential difference spectra and single beam subtracti , e spectraof HCG enzyme immunoelectrode. On the surface of electrode, IR char-acteristic spectra of HCG, anti-HCG antibody and their complex werediscussed. HCG immunoelectrode was prepared in two different waysand their spectra were compared and analyzed. The characteristic bandsof anti-HCG antibody were determined preliminarily.

关 键 词：免疫电极 FTIR 反射光谱 HCG 

分 类 号：Q579.13[生物学—生物化学]