低浓度牛血清培养Vero细胞的研究  

Study on Culture of Vero Cells with Low Concentration Calf Serum

作　　者：顾鸣[1] 邵盖斌 顾勤[1] 吴维峥 

机构地区：[1]卫生部上海生物制品研究所,上海200052

出　　处：《中国生物制品学杂志》1995年第1期28-30,共3页Chinese Journal of Biologicals

摘　　要：用人血浆组分IV中某些成分配制的低浓度牛血清（2％）培养液（称Ⅱ号培养液），培养Vero细胞的效果与美国GIBCO公司的低浓度牛血清培养基添加物Gms－A类似；用所培养的Vero细胞感染狂犬病毒，其病毒滴度均大于6．0logLD50／ml）；而残余牛血清含量从50～100μg／ml降至5～10μg／ml。The cultiration of Vero cells with low concentration calf serum prepared by authors was compared with that prepared by GIBCO (USA ) and similar results were obtained. The Vero cells cultured by both calf serum were infected with rebies .virus, and the virus titers were≥6．0OLogLD50/ml, and the residual calf serum content in culture fluid decreased from 50~100μg/ml to 5~10μg/ml.

关 键 词：低浓度 牛血清 VERO细胞 疫苗 研制 

分 类 号：R392－33[医药卫生—免疫学]