蔗糖垫超速离心法提纯乙型脑炎疫苗  

Purification of Japanese Encephalitis Vaccine by Ultracentrifugation with Sucrose Bed

作　　者：丁志芬[1] 陈景才 石慧颖[1] 常振彦[1] 李荆[1] 赵敏[1] 庞成华[1] 杨抗抗[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《中国生物制品学杂志》1995年第2期73-76,共4页Chinese Journal of Biologicals

摘　　要：经Vero细胞培养的P3株乙脑灭活病毒，以超滤浓缩和硫酸色精蛋白处理后，40％蔗糖垫层在27500r／min离心6小时，收集沉淀制成提纯乙脑疫苗。其蛋白含量在0．012～0.035mg／ml，稀释8倍后其疫苗效价仍能超过日本提纯鼠脑乙脑疫苗参考标准和我国乙脑疫苗参考标准。Vero细胞残余DNA＜100pg／0．5ml，残余牛血清＜1μg／ml。The strain P3 of inactivated Japanese encephalitis virus cultured in Vero cells was concentrated by ultrafiltration and treated by protamine sulfate, then ultracentrifuged with 40%sucrose bed at 27500rpm for 6 hours. The precipitate was collected and used for the preparation of JE vaccine. The protien content of the JE vaccine was 0.012~0. 035 mg/ml, and the vaccine titer after 8-folds dilution was stiIl higher than that of the reference standards of Japanese and Chinese JE vaccines. The residual DNA and calf serum in vaccine were <100pg/0. 5ml and <1μg/ml, respectively.

关 键 词：乙型脑炎 疫苗 提纯 蔗糖垫 超速离心 

分 类 号：R512.320.3[医药卫生—内科学] R392－33[医药卫生—临床医学]